Detection of Residual Host Cells in Sex–mismatched Bone Marrow Transplantation in Various Hematological Diseases by Fluorescence in situ Hybridization |
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| Authors: | Mansyur Arif Kimio Tanaka Tirukalikundram Subrahmanyan Kumaravel Mariko Eguchi Kohji Iwato Hiroo Dohy Nanao Kamada |
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| Affiliation: | Department of Cancer Cytogenetics, Research Institute for Radiation Biology and Medicine, Hiroshima University, 1–2–3 Kasumi, Minami–ku, Hiroshima 734;Fourth Department of Internal Medicine, Hiroshima Red Cross Hospital, 1–9–6 Senda Machi, Naka–ku, Hiroshima 730 |
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| Abstract: | Thirty–eight sex–mismatched bone marrow transplantation patients with various hematological diseases were followed–up using fluorescence in situ hybridization. Probes specific for various transloca–tions, the X chromosome (DXZ1) and the whole Y chromosome (WCPY), were used to assess successful engraftment and residual host cells. The combination of translocation and WCP Y probes enabled the identification of host and donor cells in addition to the identification of malignant vs. normal cells in the transplant recipient. Fifteen patients were sequentially followed up. The results obtained using the combination of translocation plus WCP Y probes were more reliable than those with DXZ1 plus WCP Y probes, or the translocation probe alone, especially when the percentage of residua] leukemic cells detected by the translocation probe alone was around the cut–off level. |
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| Keywords: | Residual host cells Sex mismatched BMT FISH |
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