巢式PCR在鉴别多房棘球绦虫及细粒棘球绦虫基因亚型中的应用

目的 探索巢式PCR在鉴别多房棘球绦虫及细粒棘球绦虫基因亚型中的应用价值.方法 将水泡带绦虫、犬弓首蛔虫、多房棘球绦虫、细粒棘球绦虫羊株和骆驼株等样本的DNA用巢式PCR扩增.结果 巢式PCR可以扩增出多房棘球绦虫和水泡带绦虫,而对细粒棘球绦虫羊株和骆驼株及其他寄生虫均不能扩增出.结论 在鉴别细粒棘球绦虫和多房棘球绦虫方面,巢式PCR有一定的诊断意义,但不能用于鉴别细粒棘球绦虫基因亚型.

Application of Nest PCR in Differentiating Genotype of Echinococcus granulosus and Echinococcus multilocularis

Objective To explore the role of the nest polymerase chain reaction(PCR) in differentiating genotype of Echinococcus granulosus and Echinococcus multilocularis.Methods Samples of Echinococcus granulosus sheep strain,Toxocara canis,Taenia hydatigena,Echinococcus granulosus/multilocularis mixed sample,Echinococcus sheep strain/camel strain mixed samples were used for experiment.Results The nest PCR could amplified Echinococcus multilocularis and Taenia hydatigena's DNA samples.Echinococcus sheep strain and/or camel strain and others parasites' DNA samples could not be amplified by the nest PCR.Conclusions The results show that the nest PCR may play a role in differentiation of Echinococcus granulosus and Echinococcus multilocularis.And the method could not be used in differentiation of Echinococcus granulosus genotype.