环氧合酶-2、血管内皮生长因子及上皮钙黏素在甲状腺癌中的表达及其临床意义

 目的　探讨环氧合酶-2（COX-2）、血管内皮生长因子（VEGF）、上皮钙黏素（E-cadherin）在甲状腺癌中的表达及其临床病理意义。方法　采用免疫织织化学 SP 法检测68例甲状腺癌、16例甲状腺瘤及16例正常甲状腺组织中COX-2、E-cadherin、VEGF 的表达，并对甲状腺癌组织学分型、临床病理分期及淋巴结转移进行对比分析。结果　68例甲状腺癌中COX-2、VEGF、E-cadherin的阳性表达率分别为63.2 %、66.2 %和60.3 %，和甲状腺瘤相比较差异有统计学意义（P＜0.05）。甲状腺癌中COX-2、VEGF、E-cadherin的表达在不同临床病理分期、淋巴结转移状态差异均有统计学意义（P＜0.05）。E-cadherin 与VEGF、COX-2的表达均呈负相关（r＝-0.423，P＜0.01；r＝-0.413，P＜0.01）；COX-2与VEGF 的表达呈正相关（r＝0.659，P＜0.01）。结论　COX-2、E-cadherin、VEGF 在甲状腺癌的发生、发展中起着重要作用，三者联合检测可为甲状腺癌恶性程度和预后的判断提供有效证据。

Expression and their clinical significance of COX-2, VEGF and E-cadherin in thyroid carcinoma

Objective To study the expression of COX-2,E-cadherin and VEGF in thyroid carcinoma and its clinicopathological significance.Methods Expression of COX-2,E-cadherin and VEGF was detected in 68 cases with thyroid carcinoma,16 cases with thyroid adenomas and 16 cases of normal thyroid tissue by SP immunohistochemical method.Results The expression of COX-2,E-cadherin and VEGF in 68 cases with thyroid carcinoma was 63.2%,61.8%and 66.2%,respectively,with significant difference(P<0.05)compared with the expression of these proteins in thyroid adenomas.Expression of COX-2,VEGF and E-cadherin Was significantly different with TNM stages and with lymph node metastasis status(P<0.05).Expression of E-cadherin was negatively correlated to the expression of VEGF and COX-2(r=-0.423,P<0.01;r=-0.413.P<0.01).Expression of COX-2 was positively related to the expression of VEGF(r=0.659,P<0.01). Conclusion COX-2, E-cadherin and VEGF play important roles in carcinogenesis and development of thyroid carcinoma. Combined detection can provide valuable evidence for estimation of aggressive degree and prognosis of thyroid carcinoma.