| 人癌胚抗原重组痘苗病毒转染树突状细胞体外诱导的抗肿瘤免疫 |
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| 引用本文： | 吴军,杨太成,王晓怀,杨传红,赖晃文,王捷,郑文岭. 人癌胚抗原重组痘苗病毒转染树突状细胞体外诱导的抗肿瘤免疫[J]. 中华微生物学和免疫学杂志, 2003, 23(1): 27-30 |
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| 作者姓名： | 吴军 杨太成 王晓怀 杨传红 赖晃文 王捷 郑文岭 |
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| 作者单位： | 1. 510010,广州军区广州总医院肿瘤分子生物学研究所
2. 510010,广州军区广州总医院放射肿瘤科 |
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| 摘 要： | 目的：研究人癌胚抗原重组痘苗病毒（rV-CEA)转染外周血树突状细胞（DC）后在外体诱导的抗CEA分泌性肿瘤免疫。方法：分离晚期结肠癌患者的外周血单核细胞，在体外用人重组粒－巨噬细胞集落刺激因子（GM－CSF）及白细胞介素4（IL－4）培养成DC，再用rV-CEA转染DC后激发自体T细胞，观察其体外激发自体T细胞的增殖能力及其诱导的细胞毒性T淋巴细胞（CTL）对自体CEA分泌性肿瘤细胞的杀伤活性，并与野生型痘苗病毒（W－VV）及无病毒转染的DC所激发的T细胞进行比较。结果：经rV-CEA转染的DC能显著刺激自体T细胞的增殖，其激活的T细胞对CEA分泌性自体肿瘤细胞具有特异性杀伤作用。结论：rV-CEA转染的DC可以诱导体CEA分泌性肿瘤免疫。
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| 关 键 词： | 树突状细胞 癌胚抗原 痘苗病毒 T淋巴细胞 |
| 修稿时间： | 2002-06-24 |
In vitro anti-tumor immune response induced by dendritic cells transfected with CEA-recombinant vaccinia virus |
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| WU Jun,YANG Tai-cheng,WANG Xiao-huai,YANG Chuan-hong,LAI Huang-wen,WANG Jie,ZHENG Wen-ling. In vitro anti-tumor immune response induced by dendritic cells transfected with CEA-recombinant vaccinia virus[J]. Chinese Journal of Microbiology and Immunology, 2003, 23(1): 27-30 |
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| Authors: | WU Jun YANG Tai-cheng WANG Xiao-huai YANG Chuan-hong LAI Huang-wen WANG Jie ZHENG Wen-ling |
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| Affiliation: | WU Jun,YANG Tai-cheng,WANG Xiao-huai,YANG Chuan-hong,LAI Huang-wen,WANG Jie,ZHENG Wen-ling. Institute of Molecular Oncology,Department of Oncology,General Hospital of Guangzhou Command,Guangzhou 510010,China Corresponding author: WU Jun,E-mail:junwuwang@hotmail.com |
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| Abstract: | Objective To study the in vitro CEA-specific anti-tumor immune response induced by dendritic cells (DCs) transfected with CEA-recombinant vaccinia virus (rV-CEA). Methods DCs isolated from peripheral blood monocytes of colocarcinoma patients were stimulated with recombinant human IL-4 and recombinant human granulocyte macrophage colony-stimulating factor(GM-CSF). These DCs were then transfected with rV-CEA and cocultured with autologous T cells. It was compared with the T cells induced by DCs transfected with wide type vaccinia virus (W-VV) or without virus. Results DCs transfected with rV-CEA are effective stimuli of T lymphocyte proliferation. The T cells induced by DCs transfected with rV-CEA can lyse autologous CEA-specific tumor cells. Conclusion DCs transfected with rV-CEA can elicit CEA-specific anti-tumor immune response. [ |
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| Keywords: | Dendritic cell Carcinoembryonic antigen (CEA) Vaccinia virus T lymphocyte |
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