山莨菪碱对乙酰胆碱和去甲肾上腺素引起的兔虹膜释放前列腺素的影响

本文研究了654-2对Ach及NE引起的兔虹膜释放PGE₂及6-keto-PGF_1α作用的影响。Ach及NE使虹膜释放PGs增加,654-2对Ach释放PGs的作用呈剂量依赖性抑制,当654-2浓度为3×10^-5mol/L时,Ach(5x10^-5mol/L)引起的PGE₂及6-keto-PGF_1α的释放量分别降低31%及30%。654-2浓度高于6x10^-5mol/L时显著抑制NE(5x10^-5mol/L)释放虹膜PGs的作用,当654-2浓度为3x10^-4mol/L时,使NE增加虹膜释放PGE:及6-keto-PGF_1α的量分别从62%及34%降至7.5%及4%(p<0.01)。654-2抑制PGs释放对其抗感染性休克等作用可能是有利的。

EFFECTS OF ANISODAMINE ON ACETYLCHOLINE-AND NOREPINEPHRINE-INDUCED PROSTAGLANDIN RELEASE BY RABBIT IRIS

The effects of anisodamine (654-2) on acetylcholine (Ach) and norepinephrine(NE)-induced prostaglandin(PG) release, measured by RIA, were investigated. The major products released by rabbit iris were PGE₂ and 6-keto-PGF_1α and the release of PGs increased with time of incubation. PGE₂ output was 0.43μg/g tissue after 30 min of incubation and 2 times greater than that of 6-keto-PGF_1α. The release of both PGE₂ and 6-keto-PGF_1α was enhanced by Ach and NE. The neurotransmitter-stimulated release of PGs by the iris was blocked by 654-2 in a dose-dependent manner. 654-2 at 5×10^-5 mol/L inhibited Ach-induced release of PGE₂ and 6-keto-PGF_1α by 31% and 30%, respectively. The increase in the release of PGE₂ and 6-keto-PGF_1α induced by NE at 5×10^-5 mol/L was 62% and 34%, respectively, and significantly reduced to 8% and 4%, respectively, in the presence of 654-2 at 3×10^-4 mol/L. The inhibitory effect of 654-2 on NE-induced release of PGs was not significant when the concentration of 654-2 was below 6x10^-5 mol/L. The inhibitory effects of 654-2 on neurotransmitter-induced release of PGs might contribute to its salutary effects in the treatment of septic shock.