高糖对人牙髓细胞增殖及氧化应激的影响

目的 探讨高糖对人牙髓细胞(HDPCs)增殖和氧化应激的影响,以探索高糖环境对牙髓的影响机制.方法 分离培养牙髓细胞,鉴定组织来源,实验分为4组:低糖组(葡萄糖5.5 mmol/L)、正常组(葡萄糖25 mmol/L)、高糖组(葡萄糖50mmol/L)、高渗组(与50 mmol/L组渗透压相等,并与5.5 mmol/L...

Effects of high glucose on proliferation and oxidative stress of human dental pulp cells

Objective To investigate the effect of high glucose on the proliferation and oxidative stress of human dental pulp cells(HDPCs),and to explore the influencing mechanism of high glucose environment on dental pulp.Methods Dental pulp cells were isolated and cultured,and the source of tissue was identified.The experiment was divided into four groups:low-glucose group(glucose 5.5 mmol/L),normal group(25 mmol/L),high-glucose group(50 mmol/L),and hyperosmolar group(osmotic pressure was equal to that of the 50 mmol/L group and glucose concentration was equal to that of the 5.5 mmol/L group).CCK-8 kits were used to detect the proliferation level of HDPCs after 1 day,3,and 5 days.DCFH-DA was used to detect intracellular ROS level after treatment with high glucose,the content of malondialdehyde(MDA)was measured by enzyme labeling method,and the activity of superoxide dismutase(SOD)was measured by Water-soluble tetrazolium method.Flow cytometry was used to analyze mitochondrial membrane potential in each group.Results(1)Compared with the normal group,high glucose had significant inhibitory effect on the proliferation of HDPCs(P<0.05).(2)The levels of reactive oxygen species(ROS)and malondialdehyde(MDA)increased significantly after exposure to 50 mM glucose for 72 h,and activity of superoxide dismutase(SOD)decreased.Compared with the normal group,there was a statistical difference(P<0.05).(3)Flow cytometry detection of mitochondrial membrane potential showed that the fluorescence value of high glucose group was higher than that of normal group(P<0.05).Conclusion High glucose can inhibit the proliferation of pulp cells,and lead to the decrease in mitochondrial membrane potential of HDPCs,induce oxidative stress,indicating that the high-glycemic environment reduces the vitality of dental pulp cells,which may also be a potential factor for the change of dental pulp tissues in diabetic patients.