| 萘亚胺类化合物对DNA光敏剪切作用的初步研究 |
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| 引用本文： | 朱冬晖,黄天宝.萘亚胺类化合物对DNA光敏剪切作用的初步研究[J].医学教育探索,1999(4):360-362. |
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| 作者姓名： | 朱冬晖 黄天宝 |
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| 作者单位： | [1]华东理工大学生物反应器工程国家重点实验室,生物化学研究 [2]华东理工大学药物化工研究所,生物化学研究 |
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| 基金项目： | 国家自然科学基金,上海市青年科技启明星计划,国家科委医药技术创新博士项目 |
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| 摘 要： | 研究了Ｎ－（Ｎ，Ｎ－二甲基）－乙胺－１，８－萘硫酰亚胺和Ｎ－（Ｎ，Ｎ－二甲基）－乙胺－１，８－萘酰亚胺对小牛胸腺ＤＮＡ的嵌入结合反应，并求取了嵌入常数，它们可以把质粒ｐＵＣ１９的超螺旋ＤＮＡ剪切为开环ＤＮＡ，在紫外光作用下，这种能力会得到不同程度的提高。
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| 关 键 词： | DNA 光敏剪切 嵌插络合 萘亚胺类化合物 |
Deoxyribonucleic Acid Binding and Photocleavage of Twe Novel Naphthyl Imides |
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| Zhu Donghui,Wei Dongzhi.Deoxyribonucleic Acid Binding and Photocleavage of Twe Novel Naphthyl Imides[J].Researches in Medical Education,1999(4):360-362. |
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| Authors: | Zhu Donghui Wei Dongzhi |
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| Abstract: | The intercalative binding interaction of two naphthyl imides, a N(N,Ndimethyl) ethylamine1,8naphthyl thiaimide) and b] (N(N,Ndimethyl) ethylamine1,8naphthalimide) with doublestranded calf thymus DNA has been studied, and an intrinsic binding constant has been obtained. Complexes have been found to promote cleavage of plasmid pUC19 DNA from the supercoiled form I to the open circular form II upon irradiation. The mechanism involved in the photocleavage of the plasmid by the complexes has also been established. |
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| Keywords: | DNA naphthyl imide photocleavage intercalation |
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