Survivin基因在膀胱癌中的表达及其与Bcl-2 和Caspase-3基因表达的相关性

目的探讨Survivin基因在膀胱移行细胞癌中的表达及其与Bcl-2和Caspase-3表达的相关性。方法应用免疫组化（sP法）检测40例膀胱癌组织中Survivin、Bcl-2和Caspase-3基因的表达。结果在40倒膀胱癌患者中，Survivin基因的阳性表达率为67．5％，Bcl-2和Caspase-3的阳性表达率分别为52．5％和45％。Survivin的阳性表达与膀胱癌的恶性程度、病理分级、是否复发、Bcl-2和Caspase3蛋白的阳性表达密切相关伊〈0．05），而与年龄、性别、肿瘤的临床分期无明显相关性。结论Survivin基因在膀胱癌的恶性程度、肿痛转移及预后等方面有较大的意义。Survivin基因表达抑制了Caspase-3的活性；Survivin与Bcl-2可能在调控肿瘤凋亡中的起协同作用，而若在膀胱癌中检测到Survivin和Bcl-2的表达，预示肿瘤的恶性程度较高，术后转移和复发的可能性较大，应密切观察病情进展。

Expression of Survivin gene and its relationship with expression of Bcl-2 and Caspase-3 in bladder carcinoma

[Objective] To study the expression of Survivin gene and its relationship with expression of Bcl-2 and Caspase-3 in bladder transitional cell carcinoma (BTCC). [Methods] Immunohistochemistry(SP) was performed to detect the expression of Survivin, Bcl-2 and Caspase-3 in 40 cases of BTCC. [Results] Among 40 cases of BTCC patients, the rate of expression of Survivin was 67.5 %, and the expression-rate of Bcl-2 and Caspase-3 was 52.5 % and 45 % respectively. The positive expression of survivin was closely correlated with malignant degree, pathological grade, the state of onset (whether was relapse or not), and the positive expression of Bcl-2 and Caspase-3 (P <0.05), but not with age, sex and clinical stage (P >0.05). [Conclusions] The levels of survivin are elevated in BTCC. Survivin plays an important role in BTCC grade, metastasis or prognosis. The expression of Survivin restrains the activity of Caspase-3; Survivin and Bcl-2 act synergism in regulation of apoptosis of tumor. The expression of Survivin and Bcl-2 simultaneously in bladder carcinoma indicates the malignant degree is higher and the possibility of metastasis or relapse was stronger. Therefore, the process of state of disease should be closely observed.