腹腔微环境与卵巢上皮性癌腹腔转移关系的研究

目的：探讨腹腔微环境与卵巢上皮性癌腹腔转移关系的研究。方法采用 RT-PCR 法及免疫组化法检测卵巢上皮性癌组织相应趋化因子受体及其配体的表达情况，腹膜组织相应配体的表达情况，以揭示趋化因子介导上皮性卵巢癌腹腔内转移机制。结果趋化因子受体 CXCR4在卵巢上皮性癌组织中表达明显高于其他趋化因子受体表达（P ＜0．01）；趋化因子受体 CX-CR4的配体 SDF-1在上皮性卵巢癌组织和腹膜中均高表达。结论趋化因子 SDF-1及其受体 CXCR4在卵巢癌组织中高表达，通过自分泌作用，可能刺激卵巢癌细胞的过度生长；SDF-1在腹膜组织高表达，通过旁分泌作用，可能介导表达 CXCR4的卵巢癌细胞向腹腔内转移。

Relationship between abdominal microenvironment with abdominal cavity metastasis of epithelial ovarian cancer

Objective To discuss the relationship between the abdominal microenvironment with abdominal cavity metastasis of epithelial ovarian cancer.Methods In order to reveal the mechanism of chemokines mediating epithelial ovarian cancer abdominal cavity metastasis,the expressions of chemokine receptor and its ligand in the epithelial ovarian carcinoma tissue and the peritoneal tissue were detected by RT-PCR and immunohistochemical.Results The expression of chemokine receptor CXCR4 in the epithelial ovarian carcinoma tissue was significantly higher than that of other chemokine receptors （P 〈0.01）.The CXCR4 ligand SDF-1 was high-expressed in the epithelial ovarian carcinoma tissue and the peritoneal tissue.Conclusion The CXCR4 and SDF-1 are high-ex-pressed in the ovarian cancer tissue and may stimulate the excessive growth of ovarian cancer cells by the autocrine function;SDF-1 in the peritoneal tissue is high-expressed and may mediate the ovarian cancer cells expressing CXCR4 transferred to the abdominal cavity.