| 肾小管上皮细胞炎性损伤中的转分化-获得组织干细胞潜能的研究 |
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| 引用本文： | 皮蕾,姜傥,黄彬,欧阳涓,陈培松,崔颖鹏,刘云锋,郭彩娇.肾小管上皮细胞炎性损伤中的转分化-获得组织干细胞潜能的研究[J].国际检验医学杂志,2014,0(14):15-16,1829. |
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| 作者姓名： | 皮蕾 姜傥 黄彬 欧阳涓 陈培松 崔颖鹏 刘云锋 郭彩娇 |
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| 作者单位： | 皮蕾(广州市妇女儿童医疗中心检验部,广东广州,510623)； 姜傥 (中山大学第一附属医院检验医学部,广东广州,510000)； 黄彬 (中山大学第一附属医院检验医学部,广东广州,510000)； 欧阳涓 (中山大学第一附属医院检验医学部,广东广州,510000)； 陈培松 (中山大学第一附属医院检验医学部,广东广州,510000)； 崔颖鹏 (中山大学第一附属医院检验医学部,广东广州,510000)； 刘云锋(广州市妇女儿童医疗中心检验部,广东广州,510623)；郭彩娇(广州市妇女儿童医疗中心检验部,广东广州,510623)； |
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| 摘 要： | 目的：研究肾脏纤维化过程中转分化的肾小管上皮细胞获得的组织干细胞潜能。方法体外建立局部肾素-血管紧张素（AngⅡ）系统炎性环境下肾小管上皮细胞（NRK-52E）转分化的细胞模型，观察其胚胎肾发育基因 Pax2和组织干细胞表面标志 CD133分子的表达和变化情况。结果局部高浓度 AngⅡ可刺激 NRK-52E 细胞表达 Pax2和 CD133分子，其作用呈剂量和时间依赖关系。结论炎性损伤导致肾小管上皮细胞转分化后可获得组织干细胞潜能。
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| 关 键 词： | 肾小管上皮细胞 转分化 Pax2 CD133 |
Study on transdifferentiation-acquiring tissue stem cell potency during renal tubular epithelial cells inflammatory damage |
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| Institution: | Pi Lei, Jiang Tang, Huang Bin, Ouyang Juan, Chen Peisong, Cui Yingpeng, Liu Yunfeng, Guo Caijiao (1. Department of Clinical Laboratory ,Guangzhou Municipal Women and Children Medical Center, Guangzhou, Guangdong 510623, China ; 2. Department of Laboratory Medicine, First Affiliated Hospital, Zhongshan University, Guangzhou , Guangdong 510000, China) |
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| Abstract: | Objective To study the potency of transdifferentiated renal tubular epithelial cells for acquiring the tissue stem cells during renal fibrosis.Methods The in vitro cellular model of renal tubular epithelial cells（NRK-52E）transdifferentiation under the inflammatory environment of the local renin-angiotensin （AngⅡ）system was established.The expression and change situation of the embryonic kidney developmental gene Pax2 and the tissue stem cell surface marker CD133 were observed.Results Local high concentration of AngⅡcould stimulate the NRK-52E cells to express Pax2 and CD133 molecule,its effect demonstrated the dose-and time-dependent relation.Conclusion The inflammatory damage leads to the transdifferentiated renal tubular epithelial cells po-tency to acquire the tissue stem cell. |
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| Keywords: | renal tubular epithelial cell transdifferentiation Pax2 CD133 |
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