化学发光免疫分析检测抗-HCV 抗体与 HCV-RNA 的关联研究

目的：分析实时荧光定量 PCR 检测 HCV-RNA 载量与化学发光免疫分析（CLIA）检测抗-HCV 抗体的相关性。方法抽取 CLIA 法抗-HCV 检测有反应性标本587例，采用实时荧光定量 PCR 进一步检测 HCV-RNA。结果荧光定量 PCR 检测 HCV-RNA，阴性225例，阳性362例，阳性率为61．67％，且阳性标本比例与 CLIA 法 S/CO 比值呈正相关。结论HCV-RNA阳性率与 CLIA 法 S/CO 比值呈正相关，可根据 S/CO 比值预测 HCV-RNA 结果。

Association between HCV-RNA and chemiluminescence immunoassay for detecting anti-HCV antibody

Objective To analyze the correlation of real-time fluorescent quantitation PCR（FQ-PCR）for detecting HCV-RNA loading and the chemiluminescence immunoassay（CLIA）for detecting anti-HCV antibody.Methods 587 samples of anti-HCV an-tibody positive detected by CLIA were furteher detected HCV-RNA by FQ-PCR.Results Among 587 samples of anti-HCV anti-body positive by the CLIA screening,225 samples were HCV-RNA negative and 362 samples were HCV-RNA positive detected by FQ-PCR,and the positive rate was 61 .67%,moreover,which was positively correlated with the S/CO ratio detected by CLIA.Con-clusion The positive rate of HCV-RNA is positively correlated with the S/CO ratio detected by CLIA.The result of HCV-RNA can be predicted according to the S/CO ratio.