乳糜血标本对血细胞分析仪测定结果影响因素的探讨

目的：评价乳糜血标本血浆置换前后对血细胞分析仪测定结果影响的临床应用价值。方法采集乳糜血标本22例，使用血细胞分析仪对血浆置换前后的标本进行检测，并与手工显微镜计数结果进行比对。结果非乳糜血样本 WBC、NEU、LY、MO、RBC、HGB 和 PLT 结果与置换前的原始血样本测定结果的差异无统计学意义（P ＞0．05）；在22例乳糜血样本检测中， WBC 计数分类、HGB、PLT 在置换前后结果差异有统计学意义（P ＜0．05），置换后乳糜血标本血细胞分析仪 WBC 计数分类和PLT 结果与显微镜计数法相关性较好（r2＝0．936；r2＝0．918）；在三酰甘油的干扰试验中，发现 WBC 计数分类、HGB、PLT 结果会随着三酰甘油浓度的增加而变化。结论采用血浆置换方法可以明显改善乳糜血标本对血细胞分析仪测定结果的影响，为临床提供准确的结果。

Study on influencing factors of chylemia sample on testing results of hematological analyzer

Objective To evaluate the clinical application value of influence of chylemia specimen before and after blood plasma exchange on the detection results of hematological analyzer.Methods 22 chylemia specimens were collected,then the hematological analyzer was used to test the chylemia specimens before and after blood plasma exchange,and the results were compared with those detected by the manual microscopic counting.Results The detection results of WBC,NEU,LY,MO,RBC,HGB and PLT in the non-chylemia specimens and the original blood specimens before blood plasma exchange had no statistically significant differences （P 〉0.05）;in 22 cases of chylemia specimens,the WBC count and classification,and the mean value of HGB and PLT before and after blood plasma exchange in the chylemia specimens had statistically significant differences （P 〈0.05）,the WBC count and clas-sification,and PLT mean value after blood plasma exchange in the chylemia specimens by the hematological analyzer had good corre-lation with those detectd by the microscopic counting method（r2 =0.936,r2 =0.918）.In the triglyceride interference test,the WBC count and classification,and the mean value of HGB and PLT could be changed with the increase of the triglyceride concentration. Conclusion Adopting the blood plasma exchange method could obviously improve the influence of chylemia specimens on the detec-tion results by the hematological analyzer and provide the accurate results for clinic.