| 支气管镜肺泡灌洗液改良抗酸染色法诊断菌阴性肺结核的价值探讨 |
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| 引用本文： | 张继萍,柳晓金,叶迎宾,黄秀香,郑立恒.支气管镜肺泡灌洗液改良抗酸染色法诊断菌阴性肺结核的价值探讨[J].国际检验医学杂志,2014,0(13):1702-1703. |
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| 作者姓名： | 张继萍 柳晓金 叶迎宾 黄秀香 郑立恒 |
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| 作者单位： | 张继萍 (邯郸市传染病医院肺科,河北邯郸,056002)； 柳晓金 (石家庄市第五医院检验科,河北石家庄,050000)； 叶迎宾 (邯郸市传染病医院检验科,河北邯郸,056002)； 黄秀香 (邯郸市传染病医院检验科,河北邯郸,056002)； 郑立恒 (河北省胸科医院检验科,河北石家庄,050000)； |
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| 基金项目： | 河北省卫生厅重大课题项目（20120025）。 |
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| 摘 要： | 目的：建立改良抗酸染色法，探讨纤维支气管镜肺泡灌洗液改良抗酸染色法对痰菌阴性肺结核患者的诊断价值。方法对50例痰菌阴性肺结核患者进行纤维支气管镜肺泡灌洗，应用传统、改良两种抗酸染色法对灌洗液进行检查，了解两种方法对肺结核诊断的阳性率。结果传统、改良两种抗酸染色法的阳性率分别为38％和82％（P ＜0．05）。结论改良抗酸染色法大大提高痰菌阴性肺结核患者的阳性诊断率，值得临床推广应用。
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| 关 键 词： | 纤维支气管镜 结核 肺 支气管肺泡灌洗液 |
Value of modified Ziehl-Neelsen acid-fast staining of bronchoalveolar lavage fluid in diagnosis of pulmonary tuberculosis with negative sputum |
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| Institution: | Zhang Jiping,Liu Xiaojin,Ye Yingbin,Huang Xiuxiang,Zheng Liheng (1. Department of Clinical Pulmona y Division, Handan Municipal Infectious Disease Hospital, H andan , Hebei 056002, China ; 2. Department of Clinical Laboratory, Shijiazhuang Municipal Fifth Hospital, Shijiazhuang , Hebei 050000, China 3. Department of Clinical Laboratory, Handan Municipal Infectious Disease Hospital, Handan, Hebei 056002, China ; 4. Hebei Provincial Chest Hospital, Shijiazhuang Hebei 050000,China) |
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| Abstract: | Objective To establish the modified Ziehl-Neelsen acid -fast staining method and to investigate the value of modi-fied Ziehl-Neelsen acid-fast staining method of bronchoalveolar lavage fluid in the diagnosis of pulmonary tuberculosis（TB）with negative sputum specimen.Methods 50 cases of negative sputum pulmonary TB were performed the bronchoalveolar lavage by the fiberoptic bronchoscope before the treatment,at the same time,the bronchoalveolar lavage fluids were collected and detected by the two methods of the traditional and modified Ziehl-Neelsen staining.The diagnostic positive rates were compared between the two groups.Results The positive rates of the two kinds of acid-fast staining method were 38% and 82% respectively,the difference showing statistical significance（P 〈0.05 ）.Conclusion The modified Ziehl-Neelsen acid-fast staining of bronchoalveolar lavage fluid can highly improve the positive diagnostic rate of pulmonary TB patients with negative sputum and deserves to be clinically promoted. |
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| Keywords: | fiberoptic bronchoscope tuberculosis pulmonary bronchoalveolar lavage fluid |
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