合肥地区无偿献血者核酸检测的应用研究

目的：探讨核酸检测（NAT）在血液筛查中的作用，为选择血液筛查策略提供科学依据。方法该中心在2012年1月至2013年4月对68662例无偿献血标本同步进行血清学和核酸检测；29例血清学阴性 HBV DNA 阳性样本进行乙型肝炎血清标志物测试；部分抗 HIV 阳性样本送疾控中心做确认实验。结果68662例标本中核酸单独阳性样本120例，输血残余风险为0．175％；乙型肝炎血清标志物检测，以 HBcAb 阳性模式居多；HIV 确认阳性11例，全部为核酸阳性标本。结论核酸检测能够降低输血残余风险，保证输血安全。NAT 和血清学检测相互补充，不能替代，应选择适合的筛查策略。

Application of nucleic acid test in voluntary blood donors in Hefei area

Objective To discuss the role of nucleic acid test（NAT）in blood screening to provide the scientific basis for the se-lection of blood screening strategies.Methods The serological detection and NAT were simultaneously performed on 68 662 speci-mens of voluntary blood donation from January 2012 to April 2013;29 samples of HBV DNA positive with serological HBsAg nega-tive were performed the serum HBV markers detection.Partial samples of anti-HIV antibody positive were sent to CDC for conduc-ting the confirmation test.Results Among 68 662 samples,120 cases of single NAT positive were detected out,the residual risk of blood transfusion was 0.175%.In the serum HBV markers detection,the mode of HBcAb was predominant.11 cases of HIV posi-tive were confirmed and all were the NAT positive samples.Conclusion NAT can reduce the residual risk of blood transfusion,en-sure the safety of blood transfusion.NAT and the serological detection are mutual complementation and not replaced by each other. The suitable screening strategy shold be selected.