Evolution of gentamicin and arsenite resistance acquisition in Ralstonia pickettii water isolates |
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| Authors: | Pompeyo Ferro Ivone Vaz-Moreira Célia M. Manaia |
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| Affiliation: | 1. State Key Laboratory of Infectious Disease Prevention and Control, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China;2. Department of Clinical Diagnostic Centre, The Fifth Medical Centre, Chinese PLA (People''s Liberation Army) General Hospital, Beijing, China;3. School of Medicine, Jiangsu University, Zhenjiang 212013, Jiangsu, China;4. First Affiliated Hospital of Dalian Medical University, Dalian 116011, China;5. Wuxi School of Medicine, Jiangnan University, Wuxi 214122, Jiangsu, China;1. Departamento de Microbiología, Escuela Nacional de Ciencias Biológicas-Instituto Politécnico Nacional, Cd. México, Mexico;2. Functional Genomics of Prokaryotes, Center for Genomic Sciences, National University of Mexico, Cuernavaca, Morelos, Mexico;3. Universidad Autónoma del Estado de Hidalgo, Pachuca, Hidalgo, Mexico;4. Laboratory of Integrative Immunology, Instituto Nacional de Enfermedades Respiratorias Ismael Cosío Villegas, Cd. México, Mexico;5. Departamento de Inmunología, Escuela Nacional de Ciencias Biológicas-Instituto Politécnico Nacional, Cd. México, Mexico;1. Poznań University of Life Sciences, Department of Biochemistry and Biotechnology, Dojazd 11, 60-632, Poznan, Poland;2. Poznan University of Technology, Department of Chemical Technology, Berdychowo 4, 60-965, Poznan, Poland;3. University of Natural Resources and Life Sciences, Department of Nanobiotechnology, Institute for Biologically Inspired Materials, Muthgasse 11-II, A-1090, Vienna, Austria;1. Department of Plant Pathology and Microbiology, Faculty of Agriculture, Food and Environment, The Institute of Environmental Sciences, The Hebrew University of Jerusalem, Rehovot, Israel;2. Department of Microbiology, University of Seville, Seville, Spain;1. Departamento de Biología, Facultad de Química y Biología, Universidad de Santiago de Chile (USACH), Av. Libertador Bernardo O´Higgins 3363, Estación Central, Santiago, Chile;2. Núcleo de Química y Bioquímica, Facultad de Estudios Interdisciplinarios, Universidad Mayor, Camino La Pirámide 5750, Huechuraba, Santiago, Chile;3. Programa de Biología Celular y Molecular, Instituto de Ciencias Biomédicas, Facultad de Medicina, Universidad de Chile, Independencia 1027, Santiago, Chile;1. N.D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Moscow, Russia;2. M.M. Shemyakin & Y.A. Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow, Russia;3. Moscow Institute of Physics and Technology, Dolgoprudny, Moscow Region, Russia;4. State Research Center for Applied Microbiology and Biotechnology, Obolensk, Moscow Region, Russia;5. Department of Clinical Microbiology, Christian Medical College, Vellore, 632004, Tamil Nadu, India;6. Department of Microbiology, Molecular Genetics and Immunology, University of Kansas Medical Center, Kansas City, KS, 66160, USA;7. Institute of Health and Biomedical Innovation, School of Biomedical Sciences, Faculty of Health, Queensland University of Technology, Brisbane, Australia |
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| Abstract: | Ralstonia pickettii are ubiquitous in water environments. Members of this species are frequently, but not always, resistant to both gentamicin and arsenite. Gentamicin and arsenite co-resistance and the putative molecular mechanisms were investigated. A group of 37 R. pickettii strains isolated from drinking water and hospital wastewater were characterized for gentamicin and arsenite resistance phenotypes, the number and size of plasmids, and screened for genetic elements associated with arsenite tolerance, Integrative and Conjugative Elements (ICEs), among other. The genomes of three representative strains were compared.Most gentamicin resistant (GR) isolates (32/33) were resistant to arsenite, and harbored ICE- and ars operon-related genes. These genetic elements were not detected in any of the five arsenite susceptible strains, regardless of the GR (n = 1) or gentamicin susceptibility (GS) (n = 4) phenotype. The comparison of the genomes of two GR (one resistant and one susceptible to arsenite) and one GS strains suggested that these phenotypes correspond to three phylogroups, distinguished by presence of some genes only in GR isolates, in addition to point mutations in functional genes. The presence of ICEs and ars operon-related genes suggest that arsenite resistance might have been acquired by GR lineages. |
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| Keywords: | Intrinsic antibiotic resistance Gentamicin resistance Arsenite resistance Ubiquitous bacteria Integrative and conjugative elements (ICEs) |
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