Heterogeneity in telomere length of human chromosomes |
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Authors: | Lansdorp PM; Verwoerd NP; van de Rijke FM; Dragowska V; Little MT; Dirks RW; Raap AK; Tanke HJ |
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Institution: | Terry Fox Laboratory, B.C. Cancer Agency, Vancouver, Canada. |
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Abstract: | Vertebrate chromosomes terminate in variable numbers of T2AG3 nucleotide
repeats. In order to study telomere repeats at individual chromosomes, we
developed novel, quantitative fluorescence in situ hybridization procedures
using labeled (C3TA2)3 peptide nucleic acid and digital imaging microscopy.
Telomere fluorescence intensity values from metaphase chromosomes of
cultured human hematopoietic cells decreased with the replication history
of the cells, varied up to six- fold within a metaphase, and were similar
between sister chromatid telomeres. Surprisingly, telomere fluorescence
intensity values within normal adult bone marrow metaphases did not show a
normal distribution, suggesting that a minimum number of repeats at each
telomere is required and/or maintained during normal hematopoiesis.
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