组织因子途径在灯盏花素肺部给药抗急性肺损伤的作用研究

目的从TF途径研究灯盏花素肺部给药抗内毒素（lipopolysaccharide,LPS）致大鼠ALI的作用.方法健康雄性SD大鼠120只随机分成五组（n=24）：正常组、生理盐水组、灯盏花素组、LPS组、灯盏花素＋LPS组;分别观察2、6、12 h大鼠的血浆和支气管肺泡灌洗液（BALF）中TF和TFPI的含量,计算TF/TFPI比值.结果与LPS组相比,灯盏花素＋LPS组大鼠血浆和BALF中TF含量明显下降（P〈0.05）,血浆和BALF中TFPI含量明显降低（P〈0.05）;与正常对照组和生理盐水组相比,LPS组各时间点大鼠血浆和BALF中的TF/TFPI比值显著增高（P〈0.05）,灯盏花素＋LPS组与LPS组相比,血浆和BALF中TF/TFPI比值明显降低（P〈0.05）,但是仍高于正常对照组和生理盐水组.结论灯盏花素肺部给药具有抗ALI的作用,其作用与抑制TF和TFPI的生成有关.

Role of Tissue Factor Pathway in Anti-ALI Effect of Pulmonary Delivery of Breviscapine

Objective To explore the therapeutic effect of pulmonary delivery of breviscapine on rats with acute lung injury induced by lipopolysaccharide from tissue factor pathway.Methods Healthy male adult Sprague-Dawley rats were randomly divided into five groups（n=24 in each group）：normal control group,saline group,breviscapine group,LPS group,and breviscapine＋LPS group.At 2 h,6 h and 12 h after treatment,the concentrations of TF and TFPI in BALF and plasma of rats in each group were measured and the TF/TFPI ratio was calculated.Results Compared with LPS group,the concentrations of TF and TFPI were decreased significantly（P〈0.05）.Compared with normal control group and saline group,TF/TFPI ratio in BALF and plasma in LPS group increased significantly（P〈0.05）.TF/TFPI ratio in BALF and plasma of rats in breviscapine＋LPS group was significantly lower than that in LPS group,but still higher than that in normal control group and saline group（P〈0.05）.Conclusions（1）Pulmonary delivery of Breviscapine has anti-ALI effects.（2）The anti-ALI effects of pulmonary delivery of Breviscapine are related to inhibiting the formation of TF and TFPI.