| Ebp1基因对舌鳞状细胞癌Tca8113细胞增殖的抑制作用 |
| |
| 引用本文： | 王庆,余优成,陈万涛,潘红芽,张志愿,陶凤娟. Ebp1基因对舌鳞状细胞癌Tca8113细胞增殖的抑制作用[J]. 复旦学报(医学版), 2009, 36(2): 201-205. DOI: |
| |
| 作者姓名： | 王庆 余优成 陈万涛 潘红芽 张志愿 陶凤娟 |
| |
| 作者单位： | 复旦大学附属中山医院口腔科,上海,200032;上海交通大学医学院附属第九人民医院口腔颌面外科,上海,200011 |
| |
| 基金项目： | 上海市科委重点基础研究项目 |
| |
| 摘 要： | 目的 研究异位表达ebp1基因对舌鳞状细胞癌Tca8113细胞增殖能力的影响。方法 pcDNA3.1- ebp1真核表达载体稳定转染舌鳞状细胞癌细胞系（Tca8113）。Western blot和RT-PCR验证稳定转染细胞中ebp1的表达；观察细胞生长、增殖，细胞周期改变，通过软琼脂克隆形成，裸小鼠体内成瘤等方法来评价ebp1抑制肿瘤细胞生长的作用。结果 Ebp1转染细胞的细胞生长、增殖明显受抑制，细胞周期改变，G0/G1和G2/M细胞增多，而S期细胞减少，软琼脂克隆形成率明显下降；移植小鼠模型中肿瘤平均直径和重量Tca-0细胞明显大于Tca-1细胞和Tca-3细胞。结论 Ebp1异位表达对Tca8113有多种抗肿瘤特性，可进一步探讨将ebp1基因作为舌鳞状细胞癌的治疗措施的可能性。
|
| 关 键 词： | 舌鳞状细胞癌 抑制作用 Ebp1 |
The inhibitory effects of ebp1 gene on proliferation of tongue squamous cell carcinoma cell line Tca8113 cells |
| |
| WANG Qing,YU You-cheng,CHEN Wan-tao,PAN Hong-ya,ZHANG Zhi-yuan,TAO Feng-juan. The inhibitory effects of ebp1 gene on proliferation of tongue squamous cell carcinoma cell line Tca8113 cells[J]. Fudan University Journal of Medical Sciences, 2009, 36(2): 201-205. DOI: |
| |
| Authors: | WANG Qing YU You-cheng CHEN Wan-tao PAN Hong-ya ZHANG Zhi-yuan TAO Feng-juan |
| |
| Affiliation: | WANG Qing1,YU You-cheng1,CHEN Wan-tao2,PAN Hong-ya2,ZHANG Zhi-yuan2,TAO Feng-juan1 |
| |
| Abstract: | Objective To observe the inhibitory effects of ebp1 gene on tongue squamous cell carcinoma cell line Tca8113 cells. Methods Tongue squamous cell carcinoma cell line Tca8113 was stably transfected with the full-length ebp1 cDNA sequence or the empty expression vector pcDNA3.1.The inhibitory effect was evaluated using in vitro proliferation assay,cell cycle distribution and anchorage-independent growth in soft agar as well as in vivo tumorigenicity.Stable gene transfer was verified by Western blot analysis an... |
| |
| Keywords: | Ebp1 |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
| 点击此处可从《复旦学报(医学版)》浏览原始摘要信息 |
|
点击此处可从《复旦学报(医学版)》下载免费的PDF全文 |
