浙江省急性腹泻患儿中肠道病毒检测分析研究

目的 探讨浙江省急性腹泻患儿的常见病毒组成.方法 采集5岁以下（≤5岁）急性腹泻患儿的811份粪便标本,采用ELISA方法检测A组轮状病毒（RV）,应用多重PCR方法同时检测B/C组RV、诺如病毒（NoV）、札如病毒（SaV）、肠道腺病毒（AdV）、星状病毒（AstV）,并利用RT-PCR对RV的阳性标本进行G、P分型.结果 811份粪便样本中,RV、NoV、SaV、AdV 及AstV总的阳性比例分别为25.52%、18.37%、4.44%、2.71%和1.23%;65份（8.01%）混合感染标本中以RV和NoV混合感染所占比例最大（56.92%）; 116份RV的G/P分型中,G3型（37.93%）、G1型（32.75%）和P[8]型（57.76%）是RV的优势型别,且G3/P[8]（27.59%）是混合感染最主要的组合.结论浙江省五种常见腹泻病毒及其混合感染的流行状况,为临床治疗和疾病监测提供参考价值.

Determination and analysis of enteric viruses from children with acute diarrhea in Zhejiang province

Objective To explore the common viral composition of acute diarrhea from Zhejiang province. Methods 811 stool specimens were collected from outpatient children （≤ 5 years） with acute diarrhea. They were tested by ELISA for group A rotavirus and by multiplex PCR for Group B/C rotavirus, Norovirus, Sapovirus, Adenovirus, and Astrovirus. For group A rotavirus-positive specimens, a RT-PCR was conducted to determine the G and P genotypes. Results In 811 cases, the overall proportions for rotavirus, norovirus, sapovirus, adenovirus, and astrovirus were 25.52%,18.37%,4.44%,2.71% and 1.23%, respectively. Mixed infections were detected in 65 （8.0%） of the 811 cases and rotavirus/norovirus dual infections took the largest percentage （56.92%）. G3 （37.93%）, G1 （32.75%） and P [8] were the most prevalent genotypes and G3/P[8] （27.59%） was the predominant combination in 116 RV-positive samples. Conclusion The results indicated the prevalence of the common agents of viral diarrhea （including mono-in- fection and mixed infection） in Zhejiang province, which would provide valuable reference for clinical treatment and disease surveillance.