| MiniSTR技术应用于孕妇血浆中胎儿游离DNA检测的研究 |
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| 引用本文： | 邓志辉,李茜,梁延连,高素青,邹红岩,杨宝成. MiniSTR技术应用于孕妇血浆中胎儿游离DNA检测的研究[J]. 中国输血杂志, 2008, 21(7): 498-501 |
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| 作者姓名： | 邓志辉 李茜 梁延连 高素青 邹红岩 杨宝成 |
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| 作者单位： | 深圳市血液中心,广东深圳,518035 |
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| 基金项目： | 深圳市科技计划重点项目 |
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| 摘 要： | 目的研究利用母血中胎儿游离DNA检测STR的方法。方法采集9名11—27孕周的孕妇抗凝血样并分离出血桨,提取血浆总DNA,利用ABI MiniFilerTM系统复合扩增D13S317、D7S820、D2S1338、D21S11、D16S539、D18S51、CSFIPO、FGA等8个常染色体miniSTR基因座和性别Amelogenin基因座,扩增产物经ABI 3100型基因测序仪作基因扫描检测,用GeneMapper ID 3.2软件分析结果。结果9例孕妇血浆样本中,均检出了父源性胎儿STR等位基因,D13S317等8个常染色体STR基因座中,平均检出胎儿特异等位基因3.1个/例。在性别Amelogenin基因座,Y等位基因检出的案例有5例,并且按ABI MiniFilerTM试剂盒的扩增条件,Amelogenin Y等位基因的荧光信号峰高均>50 RFU,平均占Amelogenin X等位基因峰高的8.45%;未检出Amelogenin Y等位基因的有4例,性别Amelogenin基因座的结果与出生后证实的胎儿性别相一致。结论MiniSTR技术适合于孕妇血浆中胎儿游离DNA的检测,在产前分子基因诊断中具有广泛的应用前景。
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| 关 键 词： | 短串联重复序列(STR) 短片段长度 miniSTR 胎儿游离DNA 产前诊断 |
Application of MiniSTR in the detection of fetal DNA in maternal plasma |
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| DENG Zhihui,LI Qian,LIANG Yanlian,et al.. Application of MiniSTR in the detection of fetal DNA in maternal plasma[J]. Chinese Journal of Blood Transfusion, 2008, 21(7): 498-501 |
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| Authors: | DENG Zhihui LI Qian LIANG Yanlian et al. |
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| Affiliation: | DENG Zhihui,LI Qian,LIANG Yanlian,et al.Shenzhen Blood Center,Shenzhen 518035,China |
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| Abstract: | Objective To develop an efficient method for detecting the short tandem repeat(STR) of fetal DNA in maternal plasma by miniSTR technique.Methods A total of 9 blood samples form pregnant women from 11 to 27 weeks of gestation were collected.Each isolated total plasma DNA was amplified in single multiplex using the ABI MiniFilerTM kit,which could simultaneously genotype the 9 miniSTR loci,including D13S317,D7S820,D2S1338,D21S11,D16S539,D18S51,CSFIPO,FGA and Amelogenin,and the PCR products were detected by usi... |
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| Keywords: | Short tandem repeat(STR) Short fragment size MiniSTR Cell-free fetal DNA in maternal plasma Prenatal diagnosis |
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