高压氧治疗对脊髓损伤大鼠脾脏组织病理的影响

目的：探讨高压氧治疗对脊髓损伤大鼠脾脏组织病理的影响。方法：40只SD大鼠随机数字表法分为4组：空白对照组（n=10）、脊髓损伤对照组（n=10）、高压氧处理对照组（n=10）和脊髓损伤高压氧治疗组（n=10）。脊髓损伤对照组和脊髓损伤高压氧治疗组采用改良Allen’s法制作T8～T9急性脊髓损伤大鼠模型。空白对照组和脊髓损伤对照组大鼠正常饲养。不进行高压氧处理；高压氧处理对照组和脊髓损伤高压氧治疗组大鼠每间隔24h进行1次高压氧处理，每天1次，连续14d。实验结束后，取各组大鼠脾脏标本电子显微镜下进行观察。结果：空白对照组大鼠脾细胞排列整齐、规则，结构清晰，细胞核居中，呈椭圆形。脊髓损伤对照组较空白对照组大鼠脾脏淋巴细胞、网状细胞等实质细胞弥漫性减少，胞质密度不均匀。血管内红细胞淤积，脾细胞凋亡后被胶质细胞等替代。高压氧处理对照组大鼠脾细胞间隙增宽，胞浆、胞质均匀，细胞排列相对整齐、规则，结构清晰，细胞核居中，呈椭圆形。脊髓损伤高压氧治疗组大鼠脾细胞及淋巴细胞增殖旺盛．细胞核染色深，细胞排列致密且较规则，细胞间隙小。结论：高压氧治疗对大鼠脊髓损伤后脾细胞损伤具有保护作用。

Effects of hyperbaric oxygen therapy on splenocytes in rats with spinal cord injury

Objective：To explore the effects of hyperbaric oxygen therapy on splenocytes in rats with spinal cord injury. Methods： Forty SD rats were randomly allocated to the normal control group（ group NC, n = 10）, spinal cord injury control group（ group SCI ,n = 10）, hyperbaric oxygen treatment control group （group HOT, tt = 10） and hyperbaric oxygen therapy for spinal cord injury group （group SCI-HOT, n = 10）, respectively. In groups SCI and SCI-HOT, the modified Allen＇s method was employed to construct the acute Ts-T9 spinal cord injury model in rats ,whilst hyperbaric oxygen therapy was spared in groups NC and HOT. In groups HOT and SCI-HOT, hyperbaric oxygen therapy was administered once daily every other 24 h for 14 consecutive days. The spleens of all groups were subjected to electron microscopic examination at the end of the study. Results： In group NC, the splenocytes appeared well-ordered and had a characteristic architecture, with their oval-shaped nuclei being positioned centrally to the cell. Apart from a ubiquitously reduced number of parenchymal cells（ i. e. lymphocytes and reticular cells） whose cytoplasmic density was heterogeneous, group SCI was characterized by intravascular red blood cell stagnation and a preponderance of gliocytes following splenocyte apoptosis. In group HOT, the splenocytes were noted as having a widened intercellular gap and homogeneous cytoplasm. These splenocytes were well-ordered and had a characteristic architecture, with their oval-shaped nuclei being positioned centrally. Intensive proliferation of splenocytes and lymphocytes which were densely and regularly ordered, with heavily stained nuclei and reduced intercellular gap, was demonstrated in group SCI-HOT. Conclusion： Hyperbaric oxygen therapy has a protective effect on spienocyte injury in rats with SCI.