实时荧光定量RT-PCR检测SALL4基因的临床应用研究

本研究旨在建立实时荧光定量RT-PCR（FQ RT-PCR）检测婆罗双树样基因4（sal-like4,SALL4）mRNA的方法并研究该基因在各类型白血病中表达情况.应用实时荧光定量RT-PCR技术定量检测60例急、慢性白血病患者和10例志愿者的标本SALL4基因.结果表明,SALL4基因在完全缓解（CR）组各类型白血病中不表达或低表达,与对照组相比无显著差异（P＞0.05）,而在初诊和复发组中高表达,表达量显著高于CR组（P＜0.05）,但在初诊和复发两组间表达量无显著差异（P＞0.05）,其中SALL4基因在CLL、T-ALL、M3中不表达或低表达；相关BMI-1基因与其表达规律一致,两者显著相关（r=0.825,P＜0.01）.结论：实时荧光定量RT-PCR技术检测SALL4基因具有高敏感性和高特异性等优点,可作为进一步研究SALL4基因的方法；SALL4基因有可能成为部分白血病治疗转归及白血病微小残留病（MRD）监测指标之一.

Detection of SALL4 mRNA Expression in Leukemia by Real-time Fluorescent Quantitative PCR

This study was purposed to establish a real-time fluorescent quantitative PCR （FQ-PCR） for quantifying SALIA mRNA and to investigate its expression in different types of leukemia patients. SALL4 mRNA expression were measured in 60 leukemia patients of different periods and 10 normal controls sequentially by FQ-PCR. The results showed that the expression of SALL4 mRNA in de novo leukemia patients and relapsed patients was higher than that in controls （P 〈 0.05 ）, which was significantly decreased at complete remission （CR）. In relapsed patients, the expression of SALIA mRNA increased slightly higher than that in de novo leukemia group, but the difference was not statistically significant （P 〉 0. 05 ）. However, the expression of SALL4 mRNA was low in CLL, T-ALL and AML-M3. The expression pattern of BMI-1 was same as SALL4, and the expression of BMI-1 positively correlated with that of SALL4 in leukemia （ r = 0. 825, P 〈 0. 01 ）. It is concluded that the detection of SALL4 gene expression in acute and chronic leukemia by real-time gTR-PCR displays high sensitivity and specificity. SALL4 gene may be one of indicators for monitoring the therapeutic outcome of partial leukemia and minimal residual disease.