多发性骨髓瘤患者外周血Th17细胞功能研究

本研究探讨Th17细胞及其分泌的细胞因子IL-17、IL-21在多发性骨髓瘤（MM）发生发展中所起的作用.将55例MM患者分为未缓解组（A组,30例）和缓解组（B组,25例）,健康自愿者作为对照组（C组,30例）.采用酶联免疫吸附法（ELISA）检测外周血单个核细胞（PBMNC）刺激培养上清液中IL-17、IL-21和IL-6的水平,并用流式细胞术分析PBMNC中Th17细胞的比例.结果表明,A组IL-6、IL-17及IL-21的水平与Th17细胞比例均明显高于B组和C组,差异有统计学意义（P＜0.05）；B组与C组相比,IL-6、IL-17及IL-21的水平与Th17比例差异均无统计学意义（P＞0.05）；未缓解组MM患者IL-17与IL-6的水平呈正相关（r=0.782,P＜0.05）,IL-21与IL-6的水平呈正相关（r =0.778,P＜0.05）.结论：Th17细胞可能作为启动因素参与了MM患者的免疫发病过程,并促进病情的进展.

Function of Peripheral Blood Th17 Cells in Patients with Multiple Myeloma

This study was aimed to investigate the effects of peripheral blood Th17 cells and their secreting IL-17, IL-21 in the occurrence and development of multiple myeloma（MM）. A total of 55 patients with MM were divided into non-remission group （group A ,n = 30 ）, remission group （group B, n = 25 ） ; healthy volunteers were used as control group （group C , n = 30 ）. The concentration of IL-17, IL-21 and IL-6 in the peripheral blood mononuclear cell （PBMNC） culture supernatant were determined with ELISA. The ratio of Th7 cells in PBMNC was determined by flow cytometry. The results showed that IL-17, IL-21 levels and ratio of Thl7 cells in group A were much higher than those in group B and C （ P 〈 0.05 ）, IL-17, IL-21 and the ratio of Thl7 cells between group B and group C were not significantly different （ P 〉 0. 05 ） ; IL-17 level in non-remission MM group positively correlaed with IL-6 level （ r = 0. 782, P 〈 0. 05 ）, IL-21 level in non-remission MM group positively correlaed with IL-6 level（ r = 0. 778, P 〈 0. 05 ）. It is concluded that Th17 cells as a initiating factor may be involved in the immune pathogenesis of MM patients, promoting the progress of the disease.