飞龙掌血提取物对高脂血症心肌缺血小鼠氧化损伤的影响

目的观察飞龙掌血提取物(Toddalia asiatica extract,TAE)对高脂血症心肌缺血小鼠氧化损伤的影响,初步探讨TAE对心血管保护作用的机制。方法选用雄性KM小鼠,随机分成对照组、模型组、辛伐他汀组、TAE高、低剂量组,除对照组外,其余各组均用牛奶代替水自由饮用,诱导高脂血症。同时灌胃给予相应的药物,每天一次,连续28 d。于试验结束72 h前分3次皮下注射盐酸异丙肾上腺素(isoprenaline hydrochloride,Iso),造成心肌缺血模型。检测小鼠体重、心电图、心脏和肝脏指数、心肌含水量、血清总胆固醇(total cholesterol,TC)、甘油三酯(triglyceride,TG)、高密度脂蛋白(high density lipoprotein,HDL)、低密度脂蛋白(low density lipoprotein,LDL)、超氧化物歧化酶(superoxide dismutase,SOD)、丙二醛(malondialdehyde,MDA)、谷胱甘肽过氧化酶(glutathione peroxidase,GPx)活性。结果 TAE可改善模型小鼠心肌缺血心电图变化,降低体重,抑制心脏、肝脏指数,降低心肌含水量;降低血清TC、TG、LDL-C含量,升高血清HDL-C含量;降低血清MDA含量,升高血清SOD、GPx活性。结论 TAE可能通过调节氧自由基代谢,改善氧化损伤,发挥其保护心血管的作用。

Protective effects of Toddalia asiatica extract on oxidative damages in mice with myocardial ischemia and hyperlipidemia

Objective To explore the effects of Toddalia asiatica extract(TAE)on oxidative damages in mice with myocardial ischemia and hyperlipidemia. Methods Fifty SPF male KM mice were randomly divided into 5 groups:control group,model group,simvastatin group (5 mg/kg),high-dose TAE group (400 mg/kg) and low-dose TAE group (100 mg/kg). Hyperlipidemia was induced by feeding milk for 4 weeks. At the same time,the corresponding drug was given by oral administration with 20 mL/kg for 28 d. At the end of four-week treatment, isoprenaline hydrochloride (Iso) was subcutaneously injected every 24 hours for three times. The body weight, electrocardiogram, heart and liver indexes, myocardial water content, and the serum levels of TC, TG, HDL, LDL, SOD, MDA and GPx were measured. Results TAE significantly improved the electrocardiogram changes induced by Iso and milk in the mice, weight lose, inhibited the heart and liver indexes, reduced the myocardial water content, decreased the levels of TC, TG, LDL and MDA, and increased the contents of HDL, SOD and GPx. Conclusions TAE may play an important role in cardiovascular protection by regulating oxygen free radical metabolism and improving oxidative damages.