六氟化硫脂质微泡的制备研究

目的 制备六氟化硫脂质微泡,为靶向微泡的研制奠定基础.方法 以二硬脂酰磷脂酰胆碱、聚乙二醇磷脂酰乙醇胺为膜成分,六氟化硫为气体核心,薄膜一超声法制备微泡.以SonoVue作对照,观测自制微泡的形态、粒径、浓度、pH值、渗透压等各项理化参数.在二次谐波模式下观察微泡显影体外水囊及兔肾实质,测量分析增强图像峰值灰度.结果 自制微泡和SonoVue均大小均匀,圆形,中空透亮,平均直径分别为2.25μm和2.50 μm,粒径分布分别为0.4～10 μm和0.2～10 μm,90%微泡直径分别控制在6 μm和8 μm以内.初始浓度分别为5 x 108～10 X 108/ml和1 x 108～5 x 108/ml,配置后的稳定性均为6 h.显影水囊灰度值分别为121.67±6.76和122.33±4.53(P>0.05),兔肾造影增强峰灰度分别为72.00±7.21和74.65±10.93(P>0.05).结论 脂质微泡制备成功,具有良好的理化特性及显影效果.

The preparation of sulfur hexafluoride lipid-coated microbubbles

Objective To prepare phospholipid-based sulfur hexafluoride gas-filled microbubbles, which were designed for targeting microbubbles. Methods The microbubbles composed of DSPC and mPEG2000-PE were produced by film-sonication method,and were compared with SonoVue. In the physical chemistry characteristics studies, the morphology, particle diameter, concentration, pH value and osmotic pressure were investigated. In the echogenicity studies, contrast harmonic imaging technique was used to investigate the enhancement of vitro water sac and normal rabbit kidney parenchyma. Results The lipid microbubbles were well-distributed, round with air holes. The average diameter of self-made microbubbles and SonoVue were 2.25 μm and 2.50μm respectively. The average diameter of self-made microbubbles and SonoVue distributed from 0.4 μm to 10 μm and 0.2 μm to 10 μm,and 90% were under 6 μm and 8 μm respectively,the concentration were 5 x 108～10 x 108/ml and 1 x I08～5 x 108/ml respectively with a stability of 6 hours. In vitro water sac, the gray scale of self-made lipid microbubbles and SonoVue were 121.67±6.76 and 122.33 ± 4.53 respectively( P>0.05). In normal rabbit kidney parenchyma, the peak video intensity of normal rabbit kidney parenchyma of self-made lipid microbubbles and SonoVue were 72.00 ± 7.21 and 74. 65± 10.93 respectively(P>0.05). Conclusions The lipid microbubbles have satisfactory physical chemistry characteristics and echogenicity.