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| Chromosome 11 aneusomy in esophageal cancers and precancerous lesions- an early event in neoplastic transformation： An interphase fluorescence in situ hybridization study from south India | |
| 作者姓名： | Mohan V Ponnala S Reddy HM Sistla R Jesudasan RA Ahuja YR Hasan Q |
| 作者单位： | Department of Genetics & Molecular Medicine Kamineni Hospitals LB Nagar Hyderabad AP India,Department of Genetics & Molecular Medicine Kamineni Hospitals LB Nagar Hyderabad AP India,Department of Molecular Biology Center for Cellular and Molecular Biology Uppal Road Hyderabad AP India,Department of Genetics & Molecular Medicine Kamineni Hospitals LB Nagar Hyderabad AP India,Department of Pathology Kamineni Hospitals Hyderabad AP India,Department of Molecular Biology Center for Cellular and Molecular Biology Uppal Road Hyderabad AP India,Department of Genetics Vasavi Hospital and Research Center Lakdi-ka-pul Hyderabad AP India,Department of Genetics & Molecular Medicine Kamineni Hospitals LB Nagar Hyderabad AP India,Department of Genetics Bhagwan Mahavir Medical Research Center AC Guards Hyderabad AP India |
| 基金项目： | We acknowledge help from Gastroenterology Units of Bhagwan Mahavir Medical Research Center, AC Guards, Hyderabad, 0smania General Hospital, Afzalgunj, Hyderabad and Kamineni Hospitals, LB Nagar, Hyderabad. We thank Mr. Srinivas and Mr.Mallesh, technicians of the Department of Pathology for their assistance with histopathology. |
| 摘 要： | AIM： To detect aneusomic changes with respect to chromosome 11 copy number in esophageal precancers and cancers wherein the generation of cancer-specific phenotypes is believed to be associated with specific chromosomal aneuploidies. METHODS： We performed fluorescence in situ hybridization （FISH） on esophageal tissue paraffin sections to analyze changes in chromosome 11 copy number using apotome-generated images by optical sectioning microscopy. Sections were prepared from esophageal tumor tissue, tissues showing preneoplastic changes and histologically normal tissues （control） obtained from patients referred to the clinic for endoscopic evaluation. RESULTS： Our results demonstrated that aneusomy was seen in all the cancers and preneoplastic tissues, while none of the controls showed aneusomic cells. There was no increase in aneusomy from precancers to cancers. CONCLUSION： Our results suggest that evaluation of chromosome 11 aneusomy in esophageal tissue using FISH with an appropriate signal capture-analysis system, can be used as an ancillary molecular marker predictive of early neoplastic changes. Future studies can be directed towards the genes on chromosome 11,which may play a role in the neoplastic transformation of esophageal precancerous lesions to cancers. |
| 关 键 词： | 染色体 食管癌 肿瘤 转化 |
| 收稿时间： | 2006 Oct 6 |
Chromosome 11 aneusomy in esophageal cancers and precancerous lesions--an early event in neoplastic transformation: an interphase fluorescence in situ hybridization study from south India |
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| Mohan V,Ponnala S,Reddy HM,Sistla R,Jesudasan RA,Ahuja YR,Hasan Q.Chromosome 11 aneusomy in esophageal cancers and precancerous lesions--an early event in neoplastic transformation: an interphase fluorescence in situ hybridization study from south India[J].World Journal of Gastroenterology,2007,13(4):503-508. | |
| Authors: | Mohan Vasavi Ponnala Shivani Reddy Hemakumar M Sistla Radha Jesudasan Rachel A Ahuja Yog Raj Hasan Qurratulain |
| Institution: | 1. Department of Genetics & Molecular Medicine, Kamineni Hospitals, LB Nagar, Hyderabad, AP, India 2. Department of Molecular Biology, Center for Cellular and Molecular Biology,Uppal Road, Hyderabad, AP, India 3. Department of Pathology, Kamineni Hospitals,Hyderabad, AP, India 4. Department of Genetics, Vasavi Hospital and Research Center, Lakdi-ka-pul, Hyderabad, AP, India 5. Department of Genetics & Molecular Medicine, Kamineni Hospitals, LB Nagar, Hyderabad, AP, India;Department of Genetics, Bhagwan Mahavir Medical Research Center, AC Guards, Hyderabad, AP, India |
| Abstract: | AIM: To detect aneusomic changes with respect to chromosome 11 copy number in esophageal precancers and cancers wherein the generation of cancer-specific phenotypes is believed to be associated with specific chromosomal aneuploidies. METHODS: We performed fluorescence in situ hybridization (FISH) on esophageal tissue paraffin sections to analyze changes in chromosome 11 copy number using apotome-generated images by optical sectioning microscopy. Sections were prepared from esophageal tumor tissue, tissues showing preneoplastic changes and histologically normal tissues (control) obtained from patients referred to the clinic for endoscopic evaluation. RESULTS: Our results demonstrated that aneusomy was seen in all the cancers and preneoplastic tissues, while none of the controls showed aneusomic cells. There was no increase in aneusomy from precancers to cancers. CONCLUSION: Our results suggest that evaluation of chromosome 11 aneusomy in esophageal tissue using FISH with an appropriate signal capture-analysis system, can be used as an ancillary molecular marker predictive of early neoplastic changes. Future studies can be directed towards the genes on chromosome 11,which may play a role in the neoplastic transformation of esophageal precancerous lesions to cancers. |
| Keywords: | Esophageal cancer Aneusomy Chromosome 11 Fluorescence in situ hybridization Early detection |
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