Selective elimination of mRNAs in vivo: complementary oligodeoxynucleotides promote RNA degradation by an RNase H-like activity. |
| |
| Authors: | P Dash I Lotan M Knapp E R Kandel and P Goelet |
| |
| Institution: | Howard Hughes Medical Institute, Center for Neurobiology and Behavior, Columbia University, College of Physicians and Surgeons, New York, NY. |
| |
| Abstract: | Oligodeoxynucleotides lead to translation arrest of complementary mRNAs in the wheat germ translation system by a degradation of the mRNA. In an attempt to develop an effective reverse genetic approach in vivo, we demonstrate that injection of short (15- to 30-nucleotide) oligonucleotides into Xenopus oocytes leads to complete degradation of both injected and endogenous mRNAs by means of an RNase H-like activity. |
| |
| Keywords: | |
|
|
