B-1 cells and concomitant immunity in Ehrlich tumour progression |
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Authors: | M.C. Azevedo M.C. PalosL. Osugui M.F. LaurindoD. Masutani S. NonogakiA.L.L. Bachi F.H.M. MeloM. Mariano |
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Affiliation: | Discipline of Immunology, Department of Microbiology, Immunology and Parasitology, Universidade Federal de São Paulo, São Paulo, Brazil |
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Abstract: | Concomitant immunity is a phenomenon in which a tumour-bearing host is resistant to the growth of an implanted secondary tumour. Metastases are considered to be secondary tumours that develop spontaneously during primary tumour growth, suggesting the involvement of concomitant immunity in controlling the rise of metastases. It has been demonstrated that B-1 cells, a subset of B-lymphocytes found predominantly in pleural and peritoneal cavities, not only increase the metastatic development of murine melanoma B16F10, but also are capable of differentiating into mononuclear phagocytes, modulating inflammatory responses in wound healing, in oral tolerance and in Paracoccidiose brasiliensis infections. Here, we studied B-1 cells’ participation in concomitant immunity during Ehrlich tumour progression. Our results show that B-1 cells obtained from BALB/c mice previously injected with Ehrlich tumour in the footpad were able to protect BALB/c and BALB/Xid mice against Ehrlich tumour challenge. In addition, it was demonstrated that BALB/Xid show faster tumour growth and have lost concomitant immunity, and that this state can be partially restored by reconstituting these animals with B-1 cells. However, further researches are required to establish the mechanism involving B-1 cells in Ehrlich tumour growth. |
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Keywords: | AF, ascitic fluid BSA, bovine serum albumin CD, cluster differentiation CDK, cyclin-dependent kinases CXR-4, chemokine receptor-4 DNA, deoxyribonucleic acid ETDA, ethylenediaminetetraacetic acid ERK, extracellular signal-regulated kinase FITC, fluorescein-isothiocyanate FSB, foetal serum bovine HE, haematoxylin and eosin IFN, interferon IgG, immunoglobulin G IgM, immunoglobulin M IL, interleukin IP, intraperitoneal LPS, lipopolysaccharide MHC, major histocompatibility complex MMP-9, matrix metalloproteinase-9 MUC18, adhesion molecule present on the surface of melanoma cells PB, Pacific Blue® PBS, phosphate buffered saline PCNA, proliferating cell nuclear antigen PE, phycoerythrin PerC P, peridinin chlorophyll protein RPM, rotations per minute SC, subcutaneous TAM, tumour associated macrophage TNF, tumour necrosis factor TH, T cell helper XID, X-linked immunodeficiency WT, wild type Δ, delta |
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