水华束丝藻(Aphanizomenon flos-aquae)毒素对小白鼠血液学若干生理指标的影响

为研究水华束丝藻粗提物进入小鼠血液后不同时间的毒性作用情况。通过培养水华束丝藻来提取石房蛤毒素 ,计算各种细胞的数量和成分变化来分析毒素对血液学若干生理指标的影响。结果表明 ,小白鼠在 0 5Mu mlSTX中毒后 3 0分钟、6、12、2 4小时 ,其血液中各血细胞数量发生程度不一的变化。其中 ,红细胞、血红蛋白和血小板等受影响不明显 ;而白细胞的变化则比较显著 ,约 3 0分钟时的变化最显著 ,随着时间的延长 ,其变化越不显著 ,即毒素对小白鼠血液各生理指标的影响随时间而减弱 ,2 4小时后小白鼠的血液各生理指标基本恢复到正常水平。结论　水华束丝藻粗提物中毒素和酸共同作用于小鼠血液 ,但它们作用的先后不同。pH5 3 ,0 5MU ml的STX进入小白鼠血液后 ,12h之前STX毒素的毒力作用占主导 ,而 2 4h则是酸中毒占主导

Effect of Aphanizomenon flos-aquae toxins on some blood physiological parameters in mice

In order to study the toxic effect of STX on mouse's blood with time lapse, STX from Aphanizomenon flos-aquae was collected and the effect of toxins on physiological parameters of blood was measured by analyzing changes in cells and components in blood. Results showed that the blood parameters in mice changed after 0.5, 6, 12 and 24 hours respectively when mice were exposed to 0.5 Mu/ml Saxitoxin (STX). The numbers of red blood cell, Hemoglobin and platelet had little changed, while the number of white cells showed evident change, especially within 30 minutes. The more time extended, the less change of white cells had, namely, the effect of STX on some blood physiological parameters in mice became weaker with time spent. After 24 hours, almost all the blood physiological parameters in mice recovered to the normal level. Therefore, it can be concluded that both of STX and acid had toxic effects on blood of mice. When STX (0.5Mu/ml, at pH5.3) were injected into blood, STX had the toxic effect within 12 hours, and after that, acid had.