| 结核分枝杆菌Ag85B与MPT64真核共表达质粒的构建及表达 |
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| 引用本文： | 江山,朱道银,骆旭东,陈全,蒋英.结核分枝杆菌Ag85B与MPT64真核共表达质粒的构建及表达[J].重庆医科大学学报,2004,29(3):276-278. |
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| 作者姓名： | 江山 朱道银 骆旭东 陈全 蒋英 |
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| 作者单位： | 重庆医科大学微生物学及免疫学教研室,重庆,400016;重庆医科大学微生物学及免疫学教研室,重庆,400016;重庆医科大学微生物学及免疫学教研室,重庆,400016;重庆医科大学微生物学及免疫学教研室,重庆,400016;重庆医科大学微生物学及免疫学教研室,重庆,400016 |
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| 摘 要： | 目的:构建结核分枝杆菌Ag85B和MPT64编码基因的共表达载体pBud85B-MPT.方法:将结核分枝杆菌Ag85B、MPT64基因同时克隆进多启动子共表达载体pBudCE4.1中,得到真核共表达质粒pBud85B-MPT;将pBud85B-MPT转染COS-7细胞,通过RT-PCR方法检测目的基因的表达.结果:在COS-7细胞中同时检测到Ag85B、MPT64的表达.结论:pBud85B-MPT共表达质粒构建成功,为进一步研究新型结核病疫苗奠定基础.
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| 关 键 词： | 结核分枝杆菌 Ag85B MPT 共表达载体 |
| 文章编号： | 0253-3626(2004)03-0276-03 |
| 修稿时间： | 2003年9月8日 |
Construction and expression of the eukaryotic coexpression plasmid of Mycobacterium tuberculosis Ag85B and MPT64 gene |
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| JIANG Shan,et al.Construction and expression of the eukaryotic coexpression plasmid of Mycobacterium tuberculosis Ag85B and MPT64 gene[J].Journal of Chongqing Medical University,2004,29(3):276-278. |
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| Authors: | JIANG Shan |
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| Abstract: | Objective:To construct an eukaryotic coexpression plasmid containing Mycobacterium tuberculosis Ag85B and MPT64 gene.Methods:Mycobacterium tuberculosis Ag85B and MPT64 gene were cloned into pBudCE4.1 to construct recombinant plasmid pBud85B-MPT.The recombinant plasmid was transfected into COS-7 cells,and its expression of Ag85B and MPT64 was assesed by RT-PCR.Results:Mycobacterium tuberculosis Ag85B and MPT64 were detected in transfected COS-7 cells.Conclusion:The recombinant plasmid pBud85B-MPT can express Ag85B and MPT simultaneously in COS-7 cells which provides the basis for further investigation of DNA vaccine against tuberculosis. |
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| Keywords: | Mycobacterium tuberculosis Ag85B MPT Coexpression vector |
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