葛根素注射液逆转人胃癌裸鼠原位移植瘤多药耐药性的实验研究

目的研究葛根素注射液对人胃癌裸鼠原位移植瘤多药耐药性的逆转作用,并初步探讨其机理.方法50只BALB/c无胸腺裸鼠,建立人胃腺癌耐药细胞SGC-7901/VCR原位移植耐药模型,在模型建立次日随机分为生理盐水组、5-FU组、5-FU 葛根素组、5-FU 异博定组、葛根素组,共给药3周,10周后拉颈处死.比较各组局部浸润、远处脏器(肝脾)及淋巴结转移情况,并称取瘤重计算各组抑瘤率.应用免疫组化检测各组胃癌组织中P-gl及MlRP蛋白的表达情况.结果化疗组脏器转移率均较生理盐水组明显降低,有显著性差异(P＜0.05),葛根素组与生理盐水组相比无显著性差异.5-FU 葛根素组的抑瘤率(34.25%)高于5-FU组(11.02%),P-gp、MRP蛋白表达明显低于5-FU组,P＜0.05,但远处脏器转移率与5-Fu组相比无统计学意义.而葛根素组抑瘤率、远处脏器转移率与生理盐水组比较无统计学意义,但P-gp、MRP蛋白表达与生理盐水组比较有显著差异,P＜0.05.结论葛根素能够逆转裸鼠原位移植瘤的多药耐药性,其逆转机理与促使P-gp、MRP表达减少有关.

Experimental Study on Puerarin Injection reverse multidrug Rresistance of Nude Mice of Human Gastric Carcinoma Constructed Using Orthotopic Transplantation

Objective To observe the reversion effect of puerarin injection on multidrug resistance of nude mice model of human gastric carcinoma constructed using orthotopic transplantation,and investigate its reversal mechanism. The study was carried out on the 50 BALB/c nude mice models, transplanted orthotopically tissue of human gastric carcinoma SGC7901/VCR into their stomach wall.After 24h of establishment models,they were divided rando mLy into five groups:normal saline group,5-Fu group, 5-Fu+puerarin group,5-Fu+verapamil group and puerarin group,by celiac injection for there weeks.The inhibitary rates of tumor growth were detected,and local invasiveness,the rates of metastasis in the local lymph node,liver and pleen were compared among different groups after 10 weeks.The expressions of P-gp and MRP were detected by immunohistochemical technique. The rates of visceras metastasis in chemotherapy group was lower significantly than that in normal saline group (P<0.05),but no difference was found between puerarin group and normal saline group.The inhibitary rates of puerarin+5-Fu group and 5-Fu group were 34.25% and 11.02%,compared each other,P<0.05.P-gp and MRP expression level of puerarin+5-Fu group was lower than of the 5-Fu group.The rates of metastasis in the local lymph node,liver and pleen were not significant difference between them.The direct cytotoxicity to tumors,the rates of visceras metastasis were not significant difference between puerarin group and normal saline group.But P-gp and MRP expression level of puerarin group was significantly lower than of the normal saline group (P<0.05). Conclusion] Puerarin injection could reserve the multidrug resistance(MDR) of nude mice model of human gastric carcinoma constructed using orthotopic transplantation,for which the decreased expression of P-gp and MRP maybe responsible.