胰岛素对人宫颈癌Hela细胞化疗敏感性的影响

目的探讨胰岛素能否增强人宫颈癌Hela细胞对顺铂（DDP）的化疗敏感性及其相关机制。方法体外培养人宫颈癌Hela细胞株，MTr法检测5U／L胰岛素作用3、6、9、12、15、18、21h后，联合DDP（7．26mg／L）对人宫颈癌Hela细胞增殖抑制率的影响。流式细胞仪检测对照组、DDP组、胰岛素组及联合组（胰岛素化疗前作用6h）的细胞周期时相变化情况。结果DDP组、胰岛素化疗前作用不同时间组与对照组相比，Hela细胞的增殖抑制率明显上升（P〈0．01）；且胰岛素于化疗前提前作用3、6、9、12、15h后加入DDP，宫颈癌Hela细胞的增殖抑制率明显高于DDP组（P〈0．01）。流式分析显示DDP组及胰岛素组的S期细胞比例较对照组高（P均〈0．01），联合组s期细胞比例较DDP组高（P〈0．01）。结论胰岛素具有较显著的DDP化疗增敏作用，胰岛素作用时机选择是胰岛素对宫颈癌细胞化疗增敏作用的关键。

Insulin on cisplatin sensitivity of human cervical cancer cell HeLa line

Objective To investigate the ability of insulin to enhance sensitivity to chemotherapy of human cervical cancer HeLa cells to cisplatin and its associated mechanisms. Methods The inhibition rate of giving insulin at 5 U/L at different times before combining with cisplatin at 7.26 mg/L was calculated by MTT assay. Flow cytometry was used to study the cell cycle distribution of the Hela cells of control group, DDP group, insulin group and the combination group. Results After being cultured with 5 U/L insulin for 3 h, 6 h ,9 h, 12,15 h, respectively, the inhibition rate was signifi- cantly higher than the DDP group （P 〈 0.01 ）. Flow cytometry showed insulin could increase the S phase arrest induced by DDP. Conclusion The timing of the action of insulin is the key of enhancing the sensitivity to chemotherapy of cervi- cal cancer cell line. The mechanism may be as follows ： the toxic effects of cisplatin on cervical cancer HeLa cells are as- sociated with S phase arrest, and insulin can also cause cervical cancer cells in S phase arrest, there is a synergistic effect between them.