子宫内膜癌组织中PTEN的表达及其与mTOR磷酸化的相关性研究

目的探讨子宫内膜癌组织中磷酸酶与张力蛋白同源物（phosphataseandtensinhomolog，PTEN）的表达及其对哺乳动物雷帕霉素靶蛋白（mammaliantargetofrapamycin，mTOR）磷酸化的影响。方法利用SP免疫组化法对19例正常子宫内膜组织、28例不典型增生子宫内膜组织、53例子宫内膜癌组织中PTEN、磷酸化mTOR（P—mTOR）、真核细胞翻译起始因子4E（eukaryoticinitiationfactor4E，eIF-4E）的表达情况进行检测。结果PTEN在正常子宫内膜组织、不典型增生子宫内膜组织、子宫内膜癌组织中阳性程度逐渐降低（P〈0．05），P—mTOR和eIF-4E在子宫内膜癌组织中阳性程度最高，在不典型增生子宫内膜组织和正常子宫内膜组织中阳性程度逐渐降低（P〈0．05）；PTEN与P—roTOR在子宫内膜癌组织中的表达呈负相关（r=-0．347，P=0．011），P—mTOR和elF-4E在子宫内膜癌组织中的表达呈正相关（r：0．806，P=0．000）。结论子宫内膜癌组织中存在mTOR通路蛋白异常高表达，PTEN的表达减少与mTOR的磷酸化及其下游因子eIF-4E的活化有关。

The expression of PTEN in endometrial carcinoma and its influence on activation of mTOR

Objective To investigate the expression of phosphatase and tensin homolog （PTEN） and its influence on activation of mTOR pathway. Methods In 19 cases of normal endomedium, 28 cases with endometrial atypical hy- perplasia, 53 cases of endometrial carcinoma, the expressions of PTEN, p- mTOR, eIF-4E were detected by immuno- histochemical method of SP. Results PTEN expression levels of protein in patients with endometrial carcinoma and en- dometrial atypical hyperplasia were significantly lower than those in patients with normal endometrium （ P 〈 0.05 ）. p - mTOR, eIF- 4E expression levels in patients with endometrial carcinoma and endometrial atypical hyperplasia were signif- icantly higher than those in patients with normal endometrium （ P 〈 0.05 ）. PTEN positive cases werc negatively corre- lates with p - roTOR （ r = - 0. 347, P = 0.011 ） , p - roTOR positive cases were positively correlated with eIF- 4E （ r = 0. 806,P = 0.000）. Conclusion Dysregulation of the roTOR signaling pathway plays an important role in the develop- ment of endometrial carcinoma. The mTOR phosphorylation and its downstream factor eIF- 4E activation are facilitated by reduction of PTEN.