| 分泌高效价抗碱性磷酸酶McAb杂交瘤系的建立及其在APAAP免疫组化研究中的应用 |
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| 引用本文： | 赵宁,刘雪松,黄传书,田方,金伯泉,薛采芳,陈伯虹,舒翠玲. 分泌高效价抗碱性磷酸酶McAb杂交瘤系的建立及其在APAAP免疫组化研究中的应用[J]. 中国免疫学杂志, 1991, 0(5) |
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| 作者姓名： | 赵宁 刘雪松 黄传书 田方 金伯泉 薛采芳 陈伯虹 舒翠玲 |
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| 作者单位： | 第四军医大学免疫学教研室，第四军医大学免疫学教研室，第四军医大学免疫学教研室，第四军医大学免疫学教研室，第四军医大学免疫学教研室，第四军医大学免疫学教研室，第四军医大学免疫学教研室，第四军医大学免疫学教研室 西安 710032，西安 710032，西安 710032，西安 710032，西安 710032，西安 710032，西安 710032，西安 710032 |
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| 摘 要： | 用碱性磷酸酶长程多途径免疫BALB/C小鼠,采用反向间接ELISA法筛选融合孔上清,获得2株稳定分泌抗碱性磷酸酶McAb杂交瘤细胞株,腹水效价比国外报道高15～30倍,在APAAP免疫组化检测CD抗原、病毒抗原、肿瘤抗原以及细菌菌毛抗原等研究中均获得满意结果。
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| 关 键 词： | 单克隆抗体 APAAP 杂交瘤 免疫组化技术 |
Establishment of hybridoma cell lines secreting monoclonal antibodies against alkaline phosphatase and their applications on immunohistochemistry research |
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| ZHAO ning,et al.. Establishment of hybridoma cell lines secreting monoclonal antibodies against alkaline phosphatase and their applications on immunohistochemistry research[J]. Chinese Journal of Immunology, 1991, 0(5) |
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| Authors: | ZHAO ning et al. |
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| Affiliation: | ZHAO ning,et al. Department of Immunology,The Fourth Military Medical University,Xi'an 710032 |
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| Abstract: | BALB/c mice were immunized by multi-route injections of commencial alkaline phosphatase over a perioh of three months. After 3 days of the last intravenous boost spleen cells were fused with sp2/0 cells by using PEG and hybridoma supernatants were screened by reverse ELISA. Two hybridoma cell lines secreting high titer monoclonal antibodies to alkaline phosphatase were established. Satisfactory results were obtained by alkaline phosphatase antialkaline phosphatase (APAAP) immunohistochemistry technique in detecting CD antigens, viral antigens, Tumor antigens and bacterium fimbriae antigens. |
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| Keywords: | Monoclonal antibody Hybridoma APAAP Immunohistochemistry technique |
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