高分辨质谱法测定海鱼中二噁和多氯联苯

目的　采用同位素稀释的HRGC/HRMS法定量检测鱼体中 17种 2 ,3,7,8-氯取代的二 (PCDD/Fs)和 12种二多氯联苯 (PCBs)。方法　参照美国EPA16 13及EPA16 6 8A方法 ,采用索式抽提和自动纯化系统(FMS)对鱼体中的PCDD/Fs及PCBs进行纯化和富集、用同位素稀释气相色谱与高分辨质谱联用 -多离子检测方法检测。结果　该方法可对样品中的PCDD/Fs及PCBs同系物进行有效分离 ,PCDD/Fs同位素标准物的回收率为83 83%～ 98 4 8% ,PCBs同位素标准物的回收率为 5 0 0 6 %～ 90 5 6 %。 13种PCDD/Fs在鱼体中可检出 ,总毒性当量 (TEQ)为 0 86 pg/g ,12种二PCBs在鱼体中均可检测出 ,总TEQ值为 2 77pg/ g。结论　该方法可以同时对同一样品中的 17种PCDD/Fs及 12种二PCBs进行检测 ,检测效率高 ,结果准确可靠

Determination on PCDD/Fs and Dioxin-like PCBs simultaneously in fish by isotope dilution HRGC/HRMS

Objective Seventeen PCDD/Fs and twelve dioxin-like PCBs in fish were determined by HRGC/HRMS.Methods According to EPA1663 and EPA1668A,PCDD/Fs and PCBs were extracted from fish by Soxhlet extraction,cleaned up by FMS and quantified by HRGC/HRMS-MID,using isotope dilution metholdogy.Results The congeners of PCDD/Fs and PCBs can be separated well by this method.The recovery of PCDD/Fs was 83 83%-98 48%.The recovery of PCBs was 50 06%-90 56%.Thirteen congeners of PCDD/Fs and twelve congeners of PCBs can be detected in the fish.Total Toxicity Equivalency Quantity (TEQ) of PCDD/Fs in the fish was 0 86?pg/g,TEQ of PCBs in the fish was 2 77? pg/g .Conclusion Seventeen congeners of PCDD/Fs and tweleve congeners of PCBs can be measured simultaneously in the same sample,the method was efficient and the results were accurate and reliable.