电针对缺血性脑卒中再灌注损害大鼠CREB表达的影响

The Impact of Electro-Acupuncture on the Expression of CREB in Ischemia-Reperfusion Rats

Objective：To explore the impact of electro-acupuncture on the expression of CREB in the ischemia-reperfusion rats .Meth-ods：Sixty healthy adult SD rats which were domesticated for 3 days were randomly divided into the shame group , the model group and the electro-acupuncture group .There were 20 rats in each group .The rats of the model group and the electro-acupuncture group were given the line bolt method （ MCAO） to complete the brain ischemia model , and were reperfused 2 hours after ischemia .The electro-acu-puncture group adopted the intervention of electro-acupuncture .Three days after the reperfusion , all the rats were beheaded and taken brains.The cerebral infarction volume was observed through TTC staining and image analysis , and the protein expression of CREB were detected by protein imprinting method （ Western blotting ） and gene expression of CREB were tested by Real-Time PCR.Results：TTC and the image analysis showed that the cerebral infarction volume of electro -acupuncture group was significantly smaller than that of the model group （P＆lt;0.01）;the CREB protein level in the electro-acupuncture group was obviously higher than that of the model group （P＆lt;0.01）;the CREB gene level in the electro-acupuncture group was much higher than that of the model group （P＆lt;0.01）.Conclu-sion：Electro-acupuncture can realize the improvement of the cerebral ischemia reperfusion injury by up -regulating CREB.