从溃疡性结肠炎大鼠呼吸道与肠道微生态同步动态变化探讨“肺与大肠相表里”

目的:观察溃疡性结肠炎(Ulcerative Colitis,UC)大鼠在"肠病及肺"过程中呼吸道和肠道微生态的同步动态变化情况,探讨中医"肺与大肠相表里"理论的微生态机制。方法:以三硝基苯磺酸(TNBS)-乙醇相结合的方法诱导实验性大鼠UC模型,分别在造模后第8天、第29天和第50天三个时间点对呼吸道和肠道的需氧菌总数、厌氧菌总数、肠杆菌、肠球菌、葡萄球菌、产气荚膜梭菌、双歧杆菌、乳酸杆菌进行同步检测。结果:UC大鼠出现肠道菌群失调,益生菌数量减少,条件致病菌数量增多。其呼吸道部分菌群同步出现相关变化。造模后第8天,需氧菌总数和葡萄球菌在呼吸道和肠道同步增多,厌氧菌总数和肠杆菌在肠道增多而在呼吸道减少。造模后第29天,需氧菌总数和葡萄球菌在呼吸道和肠道同步减少,厌氧菌总数和肠杆菌在肠道减少而在呼吸道增多;造模后第50天,呼吸道和肠道的需氧菌总数、厌氧菌总数和葡萄球菌在呼吸道和肠道同步增多。结论:肠病大鼠可出现呼吸道菌群的改变,在"肠病及肺"病理传变过程中,肠病大鼠呼吸道和肠道的部分菌群出现同步增多或减少的相关性变化,提示微生态菌群的变化可能是"肠病及肺"的机制和表现形式之一。

Exploration on the Interior-Exterior Relation Between Lung and Large Intestine from the Perspective of Synchronous Dynamic Change of Microecology of Respiratory and Intestinal Passage of Rats with Ulcerative Colitis

Objective：To explore the micro ecological mechanism of the TCM theory “lung and large intestine are exteriorly and interi-orly related”via observing the synchronous dynamic change of microecology of respiratory and intestinal passage of rats with ulcerative co -litis.Methods：The ulcerative colitis rat models were created by TNBS （trinitro-benzene-sulfonic acid）.We synchronously detected to-tal aerobic bacteria , anaerobic bacteria , enterobacterium , enterococcus , staphylococcus , clostridium perfringens , bifidobacterium and lactobacillus in respiratory and intestinal passage on the 8th day, the 29th day and the 50th day after molding.Results：Dysbacteriosis appeared among the rat models including probiotics decreased and conditional pathogenic bacteria increased .Meanwhile , part of the re-spiratory floras of the model rats also appeared certain relative changes .Aerobe total and staphylococcus synchronously increased both in lung and large intestine on the 8th day while anaerobe total and enterobacterium increased in intestine but decreased in lung .Aerobe to-tal and staphylococcus synchronously decreased both in lung and large intestine at the 29th day while anaerobe total and enterobacterium decreased in intestine but increased in lung .Aerobe total, anaerobe total and staphylococcus synchronously increased both in lung and large intestine at the 50th day.Conclusion：Rats with enteropathy also can suffer from changes of respiratory floras .Parts of the respira-tory and intestinal floras of rats with enteropathy appear synchronous changes during the process of enteropthy affected lung .It may in-crease or decreased synchronously .Changes in microbial floras can be one of the mechanisms and forms of “enteropathy affected lung”.