不同胆汁及其制成的胆南星对LPS诱导的急性肺损伤大鼠保护作用考察

目的:比较不同胆汁(猪、牛、羊胆汁)及其制成的胆南星对急性肺损伤大鼠的保护作用,以期为胆南星的胆汁选择和饮片分级提供参考。方法:Wistar雄性大鼠96只,随机分为空白组、模型组、猪胆汁组、牛胆汁组、羊胆汁组、猪胆南星组、牛胆南星组、羊胆南星组,各给药组大鼠每天按2. 52 g·kg^-1灌胃给予相应药液,空白组和模型组大鼠每天灌胃给予同体积生理盐水,共8 d。第8天,模型组和各给药组大鼠灌胃1 h后,腹腔注射脂多糖(LPS,2 mg·kg^-1)制备大鼠肺损伤模型,注射LPS后3,6,24 h各取4只大鼠,取血和肺组织。测定肺系数、肺含水量及肺组织湿重/干重比值(W/D);利用酶联免疫吸附法(ELISA)测定血清中的肿瘤坏死因子-α(TNF-α),白细胞介素-6(IL-6),血栓素B2(TXB2)含量,以及肺组织中超氧化物歧化酶(SOD),谷胱甘肽过氧化物酶(GSH-Px)活性和丙二醛(MDA)和基质金属蛋白酶-9(MMP-9)含量;观察大鼠肺组织病理形态,计算大鼠肺组织损伤评分。结果:与同时间点模型组比较,猪胆汁、牛胆汁、羊胆汁及其制成的胆南星给药组大鼠肺系数、肺含水量及W/D均降低,且大部分具有显著性差异(P <0. 05,P <0. 01);能够降低模型大鼠血清TNF-α,IL-6,TXB2的含量,降低肺组织中MDA和MMP-9含量,以及提高肺组织中GSH-Px和SOD活性,且大部分具有显著性差异(P <0. 05,P <0. 01)。牛胆汁组和羊胆汁组在大多数评价指标方面优于猪胆汁组,胆南星(牛胆汁制)和胆南星(羊胆汁制)在大多数评价指标方面优于胆南星组(猪胆汁制),且部分具有显著性差异(P <0. 05,P <0. 01)。结论:不同胆汁(猪、牛、羊)及其制成的胆南星均对LPS诱发的急性肺损伤大鼠具有保护作用,且牛胆汁和羊胆汁治疗效果优于猪胆汁,胆南星(牛胆汁制)和胆南星(羊胆汁制)治疗效果优于胆南星(猪胆汁制)。

Investigation of Protective Effect of Different Bile and Their Arisaema cum Bile on Acute Lung Injury Rats Induced by LPS

Objective: To compare the protective effect of different bile( porcine bile,oxgall and sheep bile) and their Arisaema cum Bile on rats with acute lung injury,so as to provide reference for the selection of bile and the classification of decoction pieces of Arisaema cum Bile. Method: Wistar male rats were randomly divided into 8 groups( n = 12),including blank group,model group,porcine bile group,oxgall group,sheep bile group,Arisaema cum Bile with porcine bile group,Arisaema cum Bile with oxgall group and Arisaema cum Bile with sheep bile group. Rats in each treatment group were given corresponding drug solution by gavage at 2. 52 g·kg^-1 every day,and rats in the model group and the blank group were given the same volume of normal saline by gavage every day for a total of 8 days. On the 8 thday,after 1 h of administration,rats in the model group and each treatment group were intraperitoneally injected lipopolysaccharide( LPS,2 mg·kg^-1) to prepare rat lung injury model. Blood and lung tissues were collected from every four rats at 3,6,24 h after LPS injection,respectively. Lung coefficient,lung water content and wet weight/dry weight ratio of lung tissue( W/D) were measured,and the levels of tumor necrosis factor( TNF)-α,interleukin( IL)-6 and thromboxane B2( TXB2) in serum and the activities of superoxide dismutase( SOD) and glutathione peroxidase( GSH-Px) and the contents of malondialdehyde( MDA) and matrix metalloproteinase-9( MMP-9) in lung tissue were determined by enzyme linked immunosorbent assay( ELISA). The pathological morphology of rat lung tissue was observed and the score of lung tissue injury was calculated. Result: Compared with the model group at the same time point,the lung coefficient,W/D,lung water content,contents of TNF-α,IL-6 and TXB2 in serum,contents of MDA and MMP-9 in lung tissue of rats in each treatment group were all decreased,and most of them had significant differences( P <0. 05,P < 0. 01),but the activities of GSH-Px and SOD were all increased,and most of them had significant differences( P < 0. 05,P < 0. 01). The oxgall group and the sheep bile group were superior to the porcine bile group in most of the indexes,the Arisaema cum Bile with oxgall group and the Arisaema cum Bile with sheep bile group were superior to the Arisaema cum Bile with porcine bile group,and some of them had significant differences( P < 0. 05,P < 0. 01). Conclusion: Each bile group and each Arisaema cum Bile group all have protective effects on rats with acute lung injury induced by LPS,and the therapeutic effect of oxgall and sheep bile is better than that of porcine bile,the therapeutic effect of Arisaema cum Bile prepared by oxgall and sheep bile is better than that of Arisaema cum Bile prepared by porcine bile.