复方槐花颗粒中槐花和侧柏叶总黄酮提取工艺的优化

目的 优选复方槐花颗粒中槐花和侧柏叶总黄酮最佳提取工艺。方法 采用超声浸提法对复方槐花颗粒中槐花和侧柏叶总黄酮进行提取,以乙醇体积分数、超声功率、超声时间和料液比为考察因素,通过单因素试验和正交优选,确定较优提取工艺,并与常规的热回流提取法进行比较研究。结果 超声浸提法最佳提取工艺条件为：用60%乙醇,在料液比1:10和超声功率40kHz条件下超声波辅助连续提取2次,每次15min,槐花和侧柏叶总黄酮提取率可达93.31%。结论 该工艺研究为复方槐花颗粒中槐花和侧柏叶总黄酮共提提供一种新方法和思路,具有耗时短、能耗低、溶剂用量少和提取效率高等优势。

Process Optimization for Extraction of Total Flavonoids from Flos Sophorae and leaves of Platycladus Orientalis in Compound Granules of Flos Sophorae

Objective To investigate an optimum extraction process of total flavonoids from Flos Sophorae and leaves of Platycladus Orientalis in compound granules of Flos Sophorae. Methods One-factor-at-a time and orthogonal array design methods were used to optimize the conditions for ultra-wave soaking extraction of total flavonoids from Flos Sophorae and leaves of Platycladus Orientalis in the compound granules of Flos Sophorae, and compared with the hot reflux method. Results The optimal conditions for extracting total flavonoids from Flos Sophorae and leaves of Platycladus Orientalis in compound granules of Flos Sophorae were 60% ethanol as the extraction solvent, solid/liquid ratio 1:10, ultrasonic power 40kHz, extraction time 15 min/time, 2 times, resulting in the extraction of total flavonoid rate was 93.31%. Conclusions The method is simple and feasible, which provided an optimum extraction process of the total flavonoids from Flos Sophorae and leaves of Platycladus Orientalis in the compound granules of Flos Sophorae.