鹅掌楸苷和光甘草定协同抗氧化作用研究

[目的] 研究鹅掌楸苷和光甘草定协同抗氧化作用。[方法] 采用1，1-二苯基-2-苦苯肼（DPPH）和2，2-联氮-二（3-乙基-苯并噻唑-6-磺酸）二铵盐（ABTS）自由基清除实验，测定鹅掌楸苷和光甘草定单独及不同浓度配比的抗氧化活性，等辐射分析法（Isobologram）对协同作用结果进行分析。[结果] 鹅掌楸苷和光甘草定配比为3∶1时对DPPH自由基清除表现为协同作用，1:1表现为相加作用，1:3表现为拮抗作用。ABTS实验结果显示复配为1:1、1:3和3:1时均有协同作用，并且复配组合中协同作用强弱为：1:3> 1:1> 3:1。[结论] 鹅掌楸苷和光甘草定按一定比例复配后具有协同抗氧化作用。

Study on synergistic antioxidant activities of Liriodendrin and Glabridin

[Objective] To evaluate the synergistic antioxidant activities of Liriodendrin and Glabridin. [Methods] The DPPH and ABTS radicals scavenging assay were established to detect the synergistic antioxidant activities of components. Isobologram method was also established to study the synergistic result. [Results] There was a synergistic antioxidant effect between Liriodendrin and Glabridin in DPPH radicals scavenging assay when the mass ratio was 3:1, addictive effect when the mass ratio was 1:1 and antagonistic effect when the mass ratio was 1:3. There was a synergistic antioxidant effect between Liriodendrin and Glabridin in ABTS radicals scavenging assay when the mass ratio was 3:1, 1:1 and 1:3. And the synergistic antioxidant effect was 1:3> 1:1> 3:1. [Conclusion] There was a synergistic antioxidant effect between Liriodendrin and Glabridin according to a certain proportion of compound.