陈皮水提物指纹图谱的化学计量学分析及与糖尿病认知功能障碍的谱效关系

目的:建立陈皮水提物的HPLC指纹图谱,并研究其与糖尿病大鼠认知功能的谱效关系。方法:采用HPLC建立21批陈皮水提物的指纹图谱,流动相乙腈(A)-0.1%的磷酸(B)梯度洗脱,检测波长270 nm;采用指纹图谱软件中的相似度评价,SPSS 24.0二维聚类分析和SIMCA 14.1主成分分析对结果进行分析;采用腹腔注射链脲佐菌素(STZ,50 mg·kg^-1)复制糖尿病大鼠模型,模型复制成功后给予陈皮水提物灌胃,定期检测空腹血糖值,诱导第12周Morris水迷宫检测实验大鼠的空间学习记忆能力,灰色关联度分析和偏最小二乘回归分析研究谱效关系。结果:确定了陈皮水提物中25个共有峰,鉴定出5种物质,并且将21个不同品种、产地的陈皮分成了2类;与模型组比较,灌胃陈皮水提物的各组大鼠空腹血糖下降,认知能力得到改善;灰色关联分析及最小偏二乘回归分析谱效关系表明,19,15,4,17,6号峰与糖尿病大鼠认知功能关联性较大。结论:不同品种、产地之间的陈皮水提物存在一定的差异,通过化学计量学可以方便、快捷的对不同产地的陈皮水提取物质进行全面的质量评价,并且陈皮水提物可降低糖尿病大鼠的空腹血糖值及改善认知能力,其发挥作用是多种有效成分共同起效的结果,其中19,15,4,17,6号峰是与糖尿病大鼠认知功能密切相关的成分。

Stoichiometric Analysis in Fingerprint of Aqueous Extract of Citri Reticulatae Pericarpium and Spectrum-effect Relationship with Diabetic Cognitive Dysfunction

Objective: To establish the HPLC fingerprint of aqueous extract of Citri Reticulatae Pericarpium,and research the spectrum-effect relationship in diabetic cognitive dysfunction rats. Method: The HPLC fingerprints of 21 batches of Citri Reticulatae Pericarpium were established,with acetonitrile (A)-0.1% of phosphoric acid (B) as the flow phase for gradient elution. The detection wavelength was 270 nm. Acomparative analysis was performed for the results based on similarity evaluation,SPSS 24.0 two-dimension clustering analysis and SIMCA 14.1 principal component analysis of fingerprint software. Thediabetic rat model wasduplicated by one-time intraperitoneal injection with streptozotocin (STZ) at the dose of 50 mg·kg^-1. After successful replication of the model,thediabetic rats were given aqueous extract of Citri Reticulatae Pericarpium by gavage. The blood glucose value was determined at regular intervals. The cognitive function was analyzed with Morris water maze experiment at the 12 week. Grey relational analysis (GRA) and partial least squares regression analysis were used to study the spectrum-effect relationship. Result: Totally 16 common peaks of water extract of Citri Reticulatae Pericarpium were confirmed,five substances were identified,and 21 species of Citri Reticulatae Pericarpium of different varieties and origins were divided into 2 categories. Compared with the model group,the blood glucose of rats in each group treated with water extract of Citri Reticulatae Pericarpiumwere reduced,and their cognitive function was improved.According to the grey correlation analysis and the least partial square regression analysis,the peaks No. 19,15,4,17 and 6 were significantly correlated with the cognitive function of diabetic rats. Conclusion: There were some differences in aqueous extract of Citri Reticulatae Pericarpium between different varieties and origins. It is convenient and fast to make a comprehensive quality evaluation of aqueous extract of Citri Reticulatae Pericarpium of different varieties and producing areas by stoichiometry,and aqueous extract of Citri Reticulatae Pericarpium can reduce the blood glucose level and improve the cognitive function of diabetic cognitive dysfunction rats,its mechanisms of action are resultedfrom the joint effects of multiple effective components,and peaks No. 19,15,4,17 and 6 are components closely related to the cognitive function of diabetic rats.