| 含日本脑炎病毒pre-M/E蛋白基因真核表达质粒的构建及免疫效果观察 |
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| 引用本文： | 张富泉,任君萍,马文煜,姜绍谆,黄庆生. 含日本脑炎病毒pre-M/E蛋白基因真核表达质粒的构建及免疫效果观察[J]. 细胞与分子免疫学杂志, 1997, 0(2) |
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| 作者姓名： | 张富泉 任君萍 马文煜 姜绍谆 黄庆生 |
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| 作者单位： | 第四军医大学微生物学教研室 |
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| 摘 要： | 本文作者将日本脑炎病毒（ＪＥＶ）的前膜蛋白和囊膜蛋白（ｐｒｅＭ／Ｅ）的基因插入到真核表达载体ｐＳＧ５中，构建了重组表达载体ｐＳＧＪＥ。用其体外转染地鼠肾细胞ＢＨＫ－２１，经间接免疫荧光法（ＩＦＡ）检测，证实了ＪＥＶ－Ｅ的瞬时表达。大量制备和纯化重组质粒，经肌肉注射免疫ＢＡＬＢ／ｃ小鼠，结果显示该重组质粒可明显促进小鼠在受到ＪＥＶ活病毒攻击后特异性抗体的产生。
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| 关 键 词： | 日本脑炎病毒 基因表达 疫苗 |
Induction of Japanese Encephalitis Virus(JEV) Specific Antibodies in Mice upon Intramuscular Injection with an Eucaryotic Expression Plasmid Containing JEV preM/E Gene |
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| Zhang Fuquan,Ren Junping,Ma Wenyu,et al.. Induction of Japanese Encephalitis Virus(JEV) Specific Antibodies in Mice upon Intramuscular Injection with an Eucaryotic Expression Plasmid Containing JEV preM/E Gene[J]. Chinese journal of cellular and molecular immunology, 1997, 0(2) |
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| Authors: | Zhang Fuquan Ren Junping Ma Wenyu et al. |
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| Abstract: | We inserted JEV preM/E gene into the eucaryotic expression plasmid pSG5. The recombinant plasmid, designated as pSGJE,was identified by in vitro transfection of BHK 21 cells. The transient expression of JEV E in BHK 21 cells was detected by indirect immunofluorescence assay(IFA) with a monoclonal antibody 2H4 against JEV E. Then we immunized BALB/c mice with purified pSGJE by intramuscular injection and the result showed that this recombinant plasmid could stimulate specific antibody response against JEV in mice. |
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| Keywords: | Japanese encephalitis virus gene expression vaccine |
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