Studies on the Subcutaneous Absorption in Mice VII: Absorption of 3H2O and 14C-sucrose from Non-buffered Solutions at Different pH Values

The possible influence on the subcutaneous absorption process of the pH of the injected unbuffered solutions was examined in normal and oestradiol pretreated mice. Eighty μl of 0.9 % NaCl, pH 4.60, 5.70, and 8.90, containing ³H₂O and ¹⁴C-sucrose in tracer concentrations were injected. In the oestradiol pretreated mice, the clearance of the tracer substance was significantly faster at pH 4.60 than at pH 8.90. Addition of hyaluronidase to the solutions abolished these differences. The results point to the connective tissue ground substance as the medium capable of modifying the absorption process according to slight pH differences of the injected solutions.