转化生长因子β_1在下颌牵张成骨过程中的表达及意义

目的:研究转化生长因子B1(TGF-B1)在下颌牵张成骨过程中的表达及意义。方法:对15只山羊行双下颌骨皮质切开术,安置口外牵张器,经7 d间歇期后,按1 mmPd的速率延长下颌骨10 mm。在间歇期末、牵张结束当天及牵张结束后第7、14和28天分别处死3只动物,取牵张区骨痂用免疫组化方法检测不同时间内TGF-B1表达水平的变化。结果:TGF-B1在下颌牵张成骨过程中呈高水平表达,主要定位于间充质细胞和成骨细胞的胞浆中,其中以牵张结束当天和第7天的表达最强,以后逐渐下降,第28天时仅有微弱表达。结论:TGF-B1可能在牵张成骨过程中, 特别是调控组织细胞应力应变信号传递中扮演十分重要的角色。

Expression of Transforming Growth Factor-beta 1 in Mandibular Distraction Osteogenesis

Objective:The purpose of this studywas to investigate the expression of TGF-B1in the regenerated bone after the operation of the mandibular osteodistraction.Methods:Bilateral mandibular osteotomies were performed in 15 goats. The mandibles were lengthened 10 mm using a custom-made distractorwith a rate of 1 mmPd. 3 goatswere killed respectively atthe end of delayed pe- riod (7 days), and 0, 7, 14 and 28 days after completion of distraction. The distracted callus from all animalswere harvested and processed for immunohistochemical detection of TGF-B1.Results:Staining for TGF-B1was localized in mesenchymal cells of the periosteal region atthe end of delay period. On 0, 7 and 14th day after completion of distraction, a marked increase inTGF-B1ex- pressionwas seen in the regenerated bone, and mainly localized in osteoblasts. On the 28th day after the distraction, TGF-B1 showed weak staining in the distracted callus.Conclusion:TGF-Bappears to play an important role in bone regeneration afterman- dibular osteodistraction.