| 微波辅助提取槐米中的槲皮素 |
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| 引用本文： | 蒙松年,陈代武.微波辅助提取槐米中的槲皮素[J].华西药学杂志,2005,20(6):561-562. |
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| 作者姓名： | 蒙松年 陈代武 |
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| 作者单位： | 邵阳医学高等专科学校,湖南,邵阳,422000;邵阳医学高等专科学校,湖南,邵阳,422000 |
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| 摘 要： | 目的优化槐米中槲皮素的提取条件。方法以槐米中槲皮素为指标,用液相色谱法测定,采用正交实验优化实验条件,对比研究了微波辅助提取法(MAE)与普通加热回流和室温浸渍法提取槐米中槲皮素的提取率。结果确定了MAE提取槐米中槲皮素的最佳条件为:粒度60~90目,水分含量为25%,功率350W,萃取时间10min。其提取时间为普通加热回流的1/12倍,室温浸渍法的1/144倍。MAE提取槐米中槲皮素提取率分别比另两种方法提高约65.5%和213.8%。结论MAE不仅提取率高,而且提取时间大大缩短,是槐米中槲皮素快速高效的提取方法。
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| 关 键 词： | 槐米 槲皮素 微波辅助提取 正交实验设计 HPLC |
| 文章编号： | 1006-0103(2005)06-0561-02 |
| 收稿时间： | 2005-03 |
| 修稿时间： | 2005年3月1日 |
Determination of quercetin in microwave assisted extraction of Flos Sophorae immaturus by HPLC |
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| MENG Song-nian,CHEN Dai-wu.Determination of quercetin in microwave assisted extraction of Flos Sophorae immaturus by HPLC[J].West China Journal of Pharmaceutical Sciences,2005,20(6):561-562. |
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| Authors: | MENG Song-nian CHEN Dai-wu |
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| Institution: | Medical College of Shaoyang, Shaoyang 422000 , China |
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| Abstract: | OBJECTIVE To optimize the extracting procedure for quercetin from Flos Sophorae immaturus.METHODS The yield of quercetin of microwave assisted extraction (MAE),heating reflux extraction and soaking extraction from Flos Sophorae immaturu were studied. HPLC was used to determine the content of quercetin in extracts. And the orthogonal experimental design was used to optimize the conditions of MAE.RESULTS The optimized conditions of MAE were as follows: the sample was 60-90 meshed,with 25% moisture and the power of microwave irradiation was 350 W,with extraction time of 20 min.CONCLUSION MAE is a rapid and efficient extraction technique for Flos Sophorae immaturus. |
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| Keywords: | Flos Sophorae immaturus Quercetin Microwave assisted extraction (MAE) Orthogonal experimental design HPLC |
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