红外光谱及色谱技术共同评价芫花条饮片及水提物的质量

目的:运用红外光谱三级鉴别技术和高效液相色谱法(HPLC)对芫花条饮片及其水提物的成分进行研究。方法:采用三级红外技术,对各批饮片及水提物进行分析研究;同时采用HPLC法测定主要药效成分西瑞香素含量。分析整理数据,确定饮片及水提物各自的特征图谱,探讨二者成分的异同。结果:一维红外可以看出,芫花条饮片和提取物分别在1 055,1 073 cm~(-1)(C-O)出现了最大吸收;在二阶导数图谱中,水提物中1 707,1 650,1 624 cm~(-1)(C=O),与西瑞香素(1 709,1 651,1 625 cm~(-1))基本一致;在二维相关红外光谱中看出,芫花条饮片和水提物各自具有3个明显的自动峰。液相含量测定结果显示,提取物中西瑞香素的含量明显高于饮片中含量。与饮片相比,芫花条水提物中香豆素类成分含量升高,表明经过水提取后该类成分达到有效富集。结论:红外三级光谱技术为芫花条饮片及其水提物提供了整体的成分信息,二维红外特征光谱可用于二者的鉴别;液相色谱测定主要活性成分的含量,进一步说明成分的变化。

Quality Evaluation of Decoction Pieces and Water Extracts of Genkwa Ramulus by Infrared Spectroscopy and Chromatography Technology

Objective: To study the decoction pieces and water extracts of Genkwa Ramulus by using high performance liquid chromatography (HPLC) and Tri-Step infrared identification method. Method: The 10 batches of crude drugs of Genkwa Ramulus were collected; their decoction pieces and water extracts were prepared separately. Tri-Step infrared spectroscopy was used to analyze various batches of decoction pieces and water extracts; HPLC was used to determine the content of daphnetin. The data were analyzed to determine the characteristic spectrum of Genkwa Ramulus decoction pieces and water extracts, and then the component differences and similarities between these two forms were investigated. Result: From the one-dimensional spectroscopy, Genkwa Ramulus decoction pieces and water extracts showed maximum absorption at 1 055 and 1 073 cm^-1 respectively. From the second derivative spectroscopy, the results of Genkwa Ramulus water extracts (1 707, 1 650 and 1 624 cm^-1) were asically consistent with those of daphnetin (1 709, 1651 and 1 625 cm^-1). Two-dimensional correlation infrared spectroscopy showed that both Genkwa Ramulus decoction pieces and water extracts had three obvious auto-peaks at different regions. From HPLC, the results showed that the content of daphnetin in the water extracts was significantly higher than that in decoction pieces, indicating that this kind of components can be effectively enriched after water extraction. Conclusion: Tri-Step infrared spectroscopy provided the overall composition information of Genkwa Ramulus decoction pieces and water extracts; two dimensional infrared spectroscopy can be used for the identification of these two forms; and HPLC was used to detect the content of main effective compositions and further explain the changes of compositions.