龟鹿二仙胶对地塞米松诱导骨髓间充质干细胞损伤的保护作用

目的 研究龟鹿二仙胶对地塞米松诱导骨髓间充质干细胞损伤的保护作用。方法 体外培养SD大鼠骨髓间充质干细胞，传至第二代后分5组培养:正常组、地塞米松（10-8mol/L）组、骨化三醇含药血清组、龟鹿二仙胶含药血清低剂量组、龟鹿二仙胶含药血清高剂量组。MTT法检测骨髓间充质干细胞活力，检测细胞上清中的超氧化物歧化酶（SOD）、丙二醛（MDA）、炎症因子白介素-6（IL-6）、小鼠白介素1β（IL-1β）、肿瘤坏死因子-α（TNF-α）。采用Western blot检测Nrf-2/HO-1/NF-κB信号通路蛋白表达。结果 龟鹿二仙胶能显著提高细胞活力，增加SOD水平、降低MDA水平、降低炎症因子水平，增加蛋白Nrf-2、HO-1蛋白表达水平，降低NF-κB信号蛋白表达。结论 龟鹿二仙胶对地塞米松诱导骨髓间充质干细胞损伤有保护作用，且作用与调控Nrf-2/HO-1/NF-κB信号相关。 

Effects and Mechanisms for Guilu Erxian Glue on Dexamethasone Induced Bone Marrow Mesenchymal Stem Cells Injury

OBJECTIVE The protective effect of Guilu Erxian Glue on dexamethasone induced bone marrow mesenchymal stem cells injury. METHODS Bone marrow mesenchymal stem cells were obtained from SD rats and divided into four groups: normal group， dexamethasone (10-8) group， low dose and high dose of Guilu Erxian Glue groups. The activity of bone marrow mesenchymal stem cells was measured by MTT assay， superoxide dismutase (SOD)， malondialdehyde (MDA)， interleukin-6 (IL-6)， IL-1β， tumor necrosis factor a (TNF-α) were measured. The expression of Nrf-2/HO-1/NF-κB signal proteins was detected by Western blot. RESULTS Guilu Erxian Glue groups can significantly improve cell vitality， increase SOD level， reduce the level of MDA， reduce the level of inflammation factor， increase the expression of Protein， Nrf-2 and HO-1 proteins， and reduce the expression of NF-κB signal proteins. CONCLUSION Guilu Erxian Glue can inhibit the injury of bone marrow mesenchymal stem cells by dexamethasone， and the role is related to the regulation of Nrf-2/HO-1/NF-κB signal.