基于网络药理学的四君子汤作用机制分析

目的:采用网络药理学方法探讨四君子汤的功效物质基础和配伍机制。方法:依托中药系统药理学分析平台(TCMSP)检索四君子汤全方4味中药的化学成分、作用靶点、相关疾病。进而构建化合物-靶点网络,靶点-疾病网络,蛋白质-蛋白质相互作用(PPI)网络,靶点-通路网络,研究四君子汤作用机制。结果:通过筛选得出27个化合物,相应靶点93个、相关疾病215种。PPI网络包含68个靶点,关键靶点涉及FOS,ESR1,AR,PPARG等。基因本体(GO)条目121个,其中生物过程相关的条目96个,分子功能相关的条目13个,细胞组成相关的条目12个。京都基因与基因组百科全书(KEGG)通路3条,涉及神经活性受体-配体相互作用信号通路、钙离子信号通路、癌症信号通路。结论:本研究结果初步验证了四君子汤的基本药理作用及其机制,并为进一步深入揭示其作用机制奠定了良好基础。

Mechanism of Si Junzitang Based on Network Pharmacology

Objective: To investigate the efficacy and compatibility mechanism of Si Junzitang. Method: Chemical components, targets and diseases related to the four traditional Chinese medicine (TCM) herbs were searched through the Traditional Chinese Medicine Systems Pharmacology Database and Analysis Platform (TCMSP). Compound-target network, target-disease network, protein-protein interaction(PPI) network, and target-pathway network were constructed to explore the mechanism of Si Junzitang. Result: Totally 27 components, 93 targets and 215 diseases related to the four TCM herbs were obtained through screening. The PPI network contains 68 targets, in which key targets involving FOS, ESR1, AR, PPARG and so on. There were 121 gene ontology(GO) entries, including 96 biological process entries, 13 molecular function entries and 12 cellar component entries. Besides, there were 3 KEGG pathways, involving Neuroactive ligand-receptor interaction, calcium signaling pathway and cancer pathway. Conclusion: The result of the study preliminarily verifies the basic pharmacological effects and related mechanisms of Si Junzitang, in order to lay a solid foundation for further studies on the mechanism of action.