基于网络药理学的参附汤作用机制分析

目的:探讨参附汤的功效物质基础和作用机制。方法:依托中药系统药理学分析平台(TCMSP)检索参附汤2味中药人参、附子的化学成分和作用靶点。进而构建化合物-靶点网络、构建蛋白互作(PPI)网络、进行基因本体(GO)功能富集分析、进行基于京都基因与基因组百科全书(KEGG)通路富集分析,研究参附汤作用机制。结果:化合物-靶点网络包含23个化合物和相应靶点118个,关键靶点涉及PTGS2,PTGS1,NCOA2,ADRB2等。PPI网络包含114个蛋白,关键蛋白涉及Akt,AP-1,p56蛋白等。GO功能富集分析得到GO条目236个,其中生物过程相关的条目191个,分子功能相关的条目20个,细胞组成相关的条目25个。KEGG通路富集筛选得到10条信号通路涉及calcium signaling pathway,neuroactive ligand-receptor interaction,apoptosis,pathways in cancer等。结论:本研究结果初步验证参附汤防治疾病的分子机制,为进一步深入探讨其作用机制提供先导信息和基础。

Mechanism of Shenfutang Based on Network Pharmacology

Objective: To investigate the efficacy and mechanism of Shenfutang. Method: The chemical components and corresponding targets related to Shenfutang were searched from the Traditional Chinese Medicine Systems Pharmacology Database and Analysis Platform (TCMSP). The compound-target network and the protein-protein interaction(PPI) network were established, and the GO functional enrichment analysis and the KEGG pathway enrichment analysis were made to explore the mechanism of Shenfutang. Result: The compound-target network contained 23 chemical components and 118 targets. The hub targets involved PTGS2, PTGS1, NCOA2, ADRB2.The PPI network contained 114 proteins, in which the hub proteins involved AKT, AP-1, p56.The GO functional enrichment analysis showed 236 GO entries (including 191 for biological process, 20 for molecular function, 25 for cellar component). The KEGG pathway enrichment analysis showed 10 pathways, involving calcium signaling pathway, neuroactive ligand-receptor interaction, apoptosis, and pathways in cancer. Conclusion: The result of the study preliminarily verifies the basic pharmacological effects and related mechanisms of Shenfutang, thus laying a solid foundation for further study on the mechanism of action.