肿瘤浸润性淋巴细胞结合偶联半乳精抗CD_3单抗趋肝性初探

目的探讨结合偶联半乳糖（Gal）的大鼠抗小鼠CD3单克隆抗体（Mouse-anti-rst—CD3monocloalantibodyAntiCD3-McAb）肿瘤浸润性淋巴细胞的（TIL）趋肝性，方法本实验把大鼠抗小鼠CD3单克隆抗体和半乳糖（Gal）偶联在一起，与3H-TdR标记TIL结合后，从尾静脉注入小鼠体内，分别在注射后不同时间抠眼取血0．5ml，然后处死，切除肝、脾、肺组织，分别称重后进行放射性强度测定。结果结果显示：注射Gal-anti-CD3-McAb-TIL小鼠肝脏组织比注射单纯TIL的小鼠肝脏组织具有较高的放射性强度（P<0.001），且该放射性持续较长一段时间，而脾、肺、血组织内放射性强度无明显差异（P>0．5P>0.5P>0．2）。结论该结果提示Gal-anti-CD3-McAb-TIL较单纯TIL具有更良好的趋肝性，本实验为肝脏恶性肿瘤生物导向治疗和靶向性基因治疗提供了新的方法。

HEPATIC-TARGETING TENDENCY ABOUT THE TILS COMBINED GAL-ANTI-CD_3

Objective For inquiring into the hepatic-targeting tendency about the TILs derived from rat HCC tumor tissuecombined Gal-anti-CD3-McAb. Methods The TILs (Labeled3H-TdR) derived from rat HCC tumor tissue combined Gal-anil-CD3 McAb and TILs (Labeled3H-TdR) alone were infused res pectively from tail vein into rat hody of two groups in this experiment. After infusion different time,the rat 0.5ml vein blood was drained from eye socket. The livers,spleens and lungs were excisedafter killing these rats,then these organs were weighed. The radio activities were measured respctively. Results The result demonstrated that the livers of rat infused TILs combined Gal-anti-CD3 McAb had much stronger radio activities than those infusedTILs alone(P<0.001). These radio activities could last for longer time also,while the radio activities in lung,speen and blood hadno obviouse difference (P>0.5P>0.5P>0.02). Conclusion This result suggests that TILs combined Gal-anti-CD3 McAb havemuch better hepatic-oriented tendency than TILs alone. This study supplys a new way for HCC biological-targeting and genetargeting therapy.